Herbert R, Marcus M, Wolff M S, Perera F P, Andrews L, Godbold J H, Rivera M, Stefanidis M, Lu X Q, Landrigan P J
Department of Community Medicine, Mount Sinai Medical Center, New York, NY 10029-6574.
IARC Sci Publ. 1990(104):205-14.
To assess the utility of DNA adducts as biomarkers of exposure to carcinogens in an industrial population, a pilot study of roofers occupationally exposed to a mixture of polycyclic aromatic hydrocarbons was conducted. DNA was isolated from peripheral white blood cells of roofers and non-occupationally exposed subjects matched for age, sex and smoking status. Occupational exposures to anthracene, fluoranthene, pyrene, benzanthracene, benzo[a]pyrene, benzo[b]fluoranthene, benzo[g,h,i]perylene and benzo[k]fluoranthene were assessed by personal breathing zone air sampling and skin wipes. Exposures to benzo[a]pyrene in air of exposed subjects ranged from 0.60 microgram/m3 to 1.39 micrograms/m3, and exposures to total polycyclic aromatic hydrocarbon (the sum of eight hydrocarbons) ranged from 6.0 micrograms/m3 to 13.8 micrograms/m3 on the day before blood collection. In the biomarker studies 10 of 12 roofers, but only 2 of 12 comparison subjects, had detectable levels of aromatic DNA adducts by 32P-postlabelling assay (p less than 0.01). The two non-roofers with detectable adducts had levels at or near the detection limit of 2 adducts per 10(9) nucleotides. In two roofer samples which were studied in a mixing experiment, the major adduct spots did not co-migrate with the guanosine N2 adduct of benzo[a]pyrene diol epoxide. These results suggest that the 32P-postlabelling assay may be useful for monitoring exposures to complex mixtures of aromatic hydrocarbons in industrial populations.
为评估DNA加合物作为工业人群接触致癌物生物标志物的效用，我们对职业性接触多环芳烃混合物的屋顶工进行了一项试点研究。从年龄、性别和吸烟状况相匹配的屋顶工以及非职业性接触者的外周血白细胞中分离出DNA。通过个人呼吸带空气采样和皮肤擦拭评估对蒽、荧蒽、芘、苯并蒽、苯并[a]芘、苯并[b]荧蒽、苯并[g,h,i]苝和苯并[k]荧蒽的职业接触情况。在采血前一天，接触者空气中苯并[a]芘的接触量范围为0.60微克/立方米至1.39微克/立方米，总多环芳烃（八种碳氢化合物之和）的接触量范围为6.0微克/立方米至13.8微克/立方米。在生物标志物研究中，12名屋顶工中有10名通过32P后标记法检测到可检测水平的芳香族DNA加合物，但12名对照受试者中只有2名检测到（p<0.01）。两名检测到加合物的非屋顶工的加合物水平处于或接近每10(9)个核苷酸2个加合物的检测限。在两项混合实验中研究的屋顶工样本中，主要加合物斑点与苯并[a]芘二醇环氧化物的鸟嘌呤N2加合物不共迁移。这些结果表明，32P后标记法可能有助于监测工业人群中对芳香烃复杂混合物的接触情况。
