Phillips D H, Schoket B, Hewer A, Grover P L
Chester Beatty Laboratories, Institute of Cancer Research, London, UK.
IARC Sci Publ. 1990(104):223-9.
32P-Postlabelling analysis has been used to detect the formation in vivo of DNA adducts by components of complex mixtures of polycyclic aromatic hydrocarbons (PAHs) in coal-tar, creosote, bitumen, juniper tar, used engine oils and fuel exhaust condensates. The presence of DNA adducts derived from these agents has been investigated in mouse skin, in human skin explants maintained in short-term organ culture and in human skin in vivo, and the formation of many different PAH-DNA adducts was observed. Similar levels and patterns of adducts were found in DNA from human skin to those in mouse skin, which is known to be susceptible to the carcinogenic activity of PAH mixtures, thus demonstrating the potential hazard to man of epidermal contact with these materials.
32P后标记分析已被用于检测煤焦油、杂酚油、沥青、杜松焦油、用过的发动机油和燃料废气冷凝物中多环芳烃（PAHs）复杂混合物的成分在体内形成的DNA加合物。已在小鼠皮肤、短期器官培养中维持的人皮肤外植体和体内人皮肤中研究了源自这些物质的DNA加合物的存在情况，并观察到许多不同PAH-DNA加合物的形成。在人皮肤DNA中发现的加合物水平和模式与已知对PAH混合物致癌活性敏感的小鼠皮肤中的相似，从而证明了表皮接触这些物质对人类的潜在危害。
