Department of Infectious Diseases, Zhoushan Hospital, Zhejiang, China.
Biosci Trends. 2011 Dec;5(6):273-6. doi: 10.5582/bst.2011.v5.6.273.
We report here a clinical and molecular study on a case suffer from severe fever with thrombocytopenia syndrome (SFTS) due to a new type of bunyavirus, named SFTS bunyavirus (SFTSV), in Zhejiang Province China. The key clinical features of this patient include fever, lymphocytopenia and thrombocytopenia. We carried out a serological and molecular investigation in the indicated case and on relatives with close contact. The SFTSV infection was confirmed through amplification of viral genetic material using the polymerase chain reaction (PCR) from the patient's serum, but not relatives with close contact. Subsequently direct sequence of PCR product demonstrated a homology of 94-96% in the nucleotide sequence compared to a reference sequence previously reported, in which the majority of patients originated from an epidemic area of Central and Northeast China. Our results suggest that SFTSV can occur in a non-epidemic area due to a similar strain of SFTSV that apparently affect the blood system, implying the importance of dissecting the pathogenesis of SFTS as well as mode of infection.
我们在此报告一例因新型布尼亚病毒（SFTS 病毒）引起的严重发热伴血小板减少综合征（SFTS）的临床和分子研究，该病毒在中国浙江省被命名为 SFTS 布尼亚病毒（SFTSV）。该患者的主要临床特征包括发热、淋巴细胞减少和血小板减少。我们对该患者及其密切接触的亲属进行了血清学和分子学调查。通过聚合酶链反应（PCR）从患者血清中扩增病毒遗传物质，证实了 SFTSV 感染，但密切接触的亲属未感染。随后，通过直接测序 PCR 产物，与先前报道的参考序列相比，核苷酸序列的同源性为 94-96%，其中大多数患者来自中国中部和东北地区的流行地区。我们的结果表明，由于明显影响血液系统的 SFTSV 类似株，SFTSV 可在非流行地区发生，提示需要深入研究 SFTS 的发病机制和感染方式。
