鉴定和描述 43 个新型多态性 EST-SSR 标记用于马蹄莲（天南星科）。
Identification and characterization of 43 novel polymorphic EST-SSR markers for arum lily, Zantedeschia aethiopica (Araceae).
机构信息
Beijing Vegetable Research Center, National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, People's Republic of China.
出版信息
Am J Bot. 2012 Dec;99(12):e493-7. doi: 10.3732/ajb.1200228. Epub 2012 Nov 28.
PREMISE OF THE STUDY
A new set of microsatellite or simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) was developed for arum lily (Zantedeschia aethiopica), which is one of the most iconic and widely recognized ornamental plants in the world. •
METHODS AND RESULTS
Using 2175 unigenes derived from 4283 random ESTs in arum lily, 166 primer pairs were designed and tested for amplification in 24 accessions from Asia, Europe, and Africa. A total of 43 loci were polymorphic, with the number of alleles per locus ranging from two to 10. The observed heterozygosity, expected heterozygosity, and polymorphism information content ranged from 0.2313 to 0.8480, 0.3034 to 0.8648, and 0.1015 to 0.7364, respectively. •
CONCLUSIONS
These novel polymorphic EST-SSR markers will facilitate future studies of genetic variation and molecular-assisted breeding systems in arum lily.
研究前提
从表达序列标签（EST）中开发了一套新的微卫星或简单重复序列（SSR）标记，用于马蹄莲（Zantedeschia aethiopica），它是世界上最具标志性和广泛认可的观赏植物之一。
方法和结果
使用来自马蹄莲的 4283 个随机 EST 中的 2175 个基因，为亚洲、欧洲和非洲的 24 个个体设计并测试了 166 对引物进行扩增。共有 43 个位点表现出多态性，每个位点的等位基因数从两个到 10 个不等。观察杂合度、期望杂合度和多态信息含量分别在 0.2313 到 0.8480、0.3034 到 0.8648 和 0.1015 到 0.7364 之间变化。
结论
这些新的多态性 EST-SSR 标记将有助于未来对马蹄莲遗传变异和分子辅助育种系统的研究。
Zotero 插件