为豆科植物二翅豆（Dipteryx alata）这一种新热带树种开发微卫星标记。
Development of microsatellite markers for the neotropical tree species Dipteryx alata (Fabaceae).
机构信息
Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia CP 131 74001-970, Goiás, Brazil.
出版信息
Am J Bot. 2012 Feb;99(2):e72-3. doi: 10.3732/ajb.1100377. Epub 2012 Jan 26.
PREMISE OF THE STUDY
Microsatellite markers were developed for the population genetic analyses of the neotropical tree Dipteryx alata (Fabaceae).
METHODS AND RESULTS
Microsatellites were developed from a genomic shotgun library. Polymorphism at each microsatellite loci was analyzed based on 94 individuals from three populations. Eight loci amplified successfully and presented one to 10 alleles, and expected heterozygosities ranged from 0.097 to 0.862. Four loci also amplified in Pterodon emarginatus and presented similar polymorphism.
CONCLUSION
The eight microsatellite primer pairs are potentially suitable for population genetic studies and successfully amplified in another Fabaceae species.
研究前提
为了对新热带树木双翅目的群体遗传分析，开发了微卫星标记。
方法和结果
从基因组散弹文库中开发了微卫星。基于来自三个群体的 94 个个体分析了每个微卫星位点的多态性。八个位点成功扩增，呈现 1 到 10 个等位基因，预期杂合度范围从 0.097 到 0.862。四个位点也在 Pterodon emarginatus 中扩增，并呈现出相似的多态性。
结论
这 8 对微卫星引物对可能适合群体遗传研究，并成功地在另一个豆科物种中扩增。
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