School of Resources and Environmental Sciences, Shanghai Key Laboratory for Ecological Processes and Restoration, East China Normal University, Shanghai 200062, China.
Am J Bot. 2011 Feb;98(2):e19-21. doi: 10.3732/ajb.1000400. Epub 2010 Dec 23.
We developed polymorphic microsatellite primers in Castanopsis sclerophylla (Lindley & Paxton) Schottky (Fagaceae), a dominant canopy tree, to provide markers for further studies on the genetic structure and mating system of this species.
Ten polymorphic microsatellite loci were isolated and successfully amplified in three C. sclerophylla populations (Huangshanjian, Shilin, and Guanmiao) from Chun'an, Zhejiang Province, China. The number of alleles per locus in these populations ranged from 3 to 17. The observed and expected heterozygosities were 0.100-0.977 and 0.294-0.916, respectively.
These microsatellite loci displayed moderate or high levels of polymorphism within the examined populations, showing the utility of primers in studying the genetic variation, parentage, and mating system of C. sclerophylla.
我们在壳斗科（山毛榉科）麻栎（Lindley & Paxton）Schottky 中开发了多态性微卫星引物，这是一种优势林冠树种，为进一步研究该物种的遗传结构和交配系统提供了标记。
从中国浙江省淳安县的黄山涧、石林和关庙三个种群中分离出并成功扩增了 10 个多态性微卫星位点。这些种群中每个位点的等位基因数从 3 到 17 不等。观察到的和预期的杂合度分别为 0.100-0.977 和 0.294-0.916。
这些微卫星位点在被研究的种群中表现出中等或高水平的多态性，表明引物在研究麻栎的遗传变异、亲子关系和交配系统方面的适用性。
