• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脂多糖在胸膜肺炎放线杆菌黏附猪气管环中的作用。

Role of lipopolysaccharides in adherence of Actinobacillus pleuropneumoniae to porcine tracheal rings.

作者信息

Bélanger M, Dubreuil D, Harel J, Girard C, Jacques M

机构信息

Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.

出版信息

Infect Immun. 1990 Nov;58(11):3523-30. doi: 10.1128/iai.58.11.3523-3530.1990.

DOI:10.1128/iai.58.11.3523-3530.1990
PMID:2228223
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC313692/
Abstract

The ability of 17 Actinobacillus pleuropneumoniae isolates, representing serotypes 1, 2, 5, and 7, to adhere to tracheal rings maintained in culture was examined. Porcine tracheal rings were infected, and 8 h after inoculation, adherent bacterial cells were evaluated. A. pleuropneumoniae adhered to tracheal rings, and marked variations were observed between and even within serotypes, suggesting that adherence of this microorganism is not primarily related to the serotype of the isolate. No relationship was found between adherence to porcine tracheal rings and plasmid profiles, virulence in mice, hemagglutination, capsular material thickness, or whole-cell protein profiles. On the other hand, we observed that all isolates of serotypes 1 and 5 had a semirough-type lipopolysaccharide (LPS), whereas isolates of serotypes 2 and 7 had a smooth-type LPS (75%) or a semirough-type LPS (25%). Results showed that 83% of isolates with a smooth-type LPS adhered in large numbers to tracheal rings, whereas 80% of isolates with a semirough-type LPS adhered poorly (P less than 0.007). Our data indicated that the degree of adherence of A. pleuropneumoniae to porcine tracheal rings appeared to be related, at least in part, to LPS profiles. Furthermore, LPS seemed to be the adhesin of A. pleuropneumoniae, since purified LPS blocked adherence of this microorganism to porcine tracheal rings.

摘要

检测了17株胸膜肺炎放线杆菌分离株（代表血清型1、2、5和7）黏附于培养的气管环的能力。用猪气管环进行感染，接种8小时后，对黏附的细菌细胞进行评估。胸膜肺炎放线杆菌可黏附于气管环，并且在血清型之间甚至血清型内部都观察到显著差异，这表明该微生物的黏附主要与分离株的血清型无关。未发现黏附于猪气管环的能力与质粒图谱、对小鼠的毒力、血凝反应、荚膜物质厚度或全细胞蛋白图谱之间存在关联。另一方面，我们观察到血清型1和5的所有分离株都具有半粗糙型脂多糖（LPS），而血清型2和7的分离株具有光滑型LPS（75%）或半粗糙型LPS（25%）。结果显示，83%具有光滑型LPS的分离株大量黏附于气管环，而80%具有半粗糙型LPS的分离株黏附能力较差（P小于0.007）。我们的数据表明，胸膜肺炎放线杆菌对猪气管环的黏附程度似乎至少部分与LPS图谱有关。此外，LPS似乎是胸膜肺炎放线杆菌的黏附素，因为纯化的LPS可阻断该微生物对猪气管环的黏附。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6195/313692/9eb75db17838/iai00059-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6195/313692/6172c0a82e5c/iai00059-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6195/313692/5073a4d8dff6/iai00059-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6195/313692/9eb75db17838/iai00059-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6195/313692/6172c0a82e5c/iai00059-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6195/313692/5073a4d8dff6/iai00059-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6195/313692/9eb75db17838/iai00059-0071-a.jpg

相似文献

1
Role of lipopolysaccharides in adherence of Actinobacillus pleuropneumoniae to porcine tracheal rings.脂多糖在胸膜肺炎放线杆菌黏附猪气管环中的作用。
Infect Immun. 1990 Nov;58(11):3523-30. doi: 10.1128/iai.58.11.3523-3530.1990.
2
Adherence of Actinobacillus pleuropneumoniae to porcine tracheal epithelial cells and frozen lung sections.
Vet Microbiol. 1991 Apr;27(2):133-43. doi: 10.1016/0378-1135(91)90004-y.
3
Inhibition of adherence of Actinobacillus pleuropneumoniae to porcine respiratory tract cells by monoclonal antibodies directed against LPS and partial characterization of the LPS receptors.
Curr Microbiol. 1999 Dec;39(6):313-0320. doi: 10.1007/s002849900465.
4
Affinity for porcine respiratory tract mucus is found in some isolates of Actinobacillus pleuropneumoniae.胸膜肺炎放线杆菌的一些分离株对猪呼吸道黏液具有亲和力。
FEMS Microbiol Lett. 1992 Oct 1;76(1-2):119-25. doi: 10.1111/j.1574-6968.1992.tb05450.x.
5
Isolation and characterization of LPS mutants of Actinobacillus pleuropneumoniae serotype 1.胸膜肺炎放线杆菌1型脂多糖突变体的分离与鉴定
Curr Microbiol. 1997 Sep;35(3):139-44. doi: 10.1007/s002849900227.
6
High-molecular-mass lipopolysaccharides are involved in Actinobacillus pleuropneumoniae adherence to porcine respiratory tract cells.高分子量脂多糖参与胸膜肺炎放线杆菌对猪呼吸道细胞的黏附。
Infect Immun. 1994 Aug;62(8):3311-9. doi: 10.1128/iai.62.8.3311-3319.1994.
7
Proteins found within porcine respiratory tract secretions bind lipopolysaccharides of Actinobacillus pleuropneumoniae.在猪呼吸道分泌物中发现的蛋白质可结合胸膜肺炎放线杆菌的脂多糖。
Infect Immun. 1994 Mar;62(3):868-73. doi: 10.1128/iai.62.3.868-873.1994.
8
Isolation and characterization of mini-Tn10 lipopolysaccharide mutants of Actinobacillus pleuropneumoniae serotype 1.胸膜肺炎放线杆菌1型mini-Tn10脂多糖突变体的分离与鉴定
Can J Microbiol. 1999 Dec;45(12):1017-26. doi: 10.1139/w99-107.
9
Adherence of Actinobacillus pleuropneumoniae to primary cultures of porcine lung epithelial cells.胸膜肺炎放线杆菌对猪肺上皮细胞原代培养物的黏附
Vet Microbiol. 2003 May 19;93(2):133-44. doi: 10.1016/s0378-1135(03)00020-8.
10
Atypical Actinobacillus pleuropneumoniae isolates that share antigenic determinants with both serotypes 1 and 7.与血清型1和7均具有共同抗原决定簇的非典型胸膜肺炎放线杆菌分离株。
J Vet Diagn Invest. 2000 Sep;12(5):444-9. doi: 10.1177/104063870001200509.

引用本文的文献

1
Temporal and Serotypic Dynamics of in South African Porcine Populations: A Retrospective Study from 1985 to 2023.南非猪群中[具体病原体未给出]的时间和血清型动态：一项1985年至2023年的回顾性研究
Pathogens. 2024 Jul 20;13(7):599. doi: 10.3390/pathogens13070599.
2
, surface proteins and virulence: a review.表面蛋白与毒力：综述
Front Vet Sci. 2023 Nov 30;10:1276712. doi: 10.3389/fvets.2023.1276712. eCollection 2023.
3
Activation of Porcine Alveolar Macrophages by Actinobacillus pleuropneumoniae Lipopolysaccharide via the Toll-Like Receptor 4/NF-κB-Mediated Pathway.

本文引用的文献

1
Toxicity of Haemophilus pleuropneumoniae for porcine lung macrophages, peripheral blood monocytes, and testicular cells.胸膜肺炎放线杆菌对猪肺巨噬细胞、外周血单核细胞和睾丸细胞的毒性
Infect Immun. 1981 Sep;33(3):673-6. doi: 10.1128/iai.33.3.673-676.1981.
2
Characterization of the hemolysin produced by haemophilus pleuropneumoniae.胸膜肺炎放线杆菌产生的溶血素的特性分析。
Am J Vet Res. 1983 Feb;44(2):344-7.
3
Identification and serotyping of Haemophilus pleuropneumoniae by coagglutination test.用协同凝集试验对猪胸膜肺炎放线杆菌进行鉴定及血清分型
猪肺泡巨噬细胞被胸膜肺炎放线杆菌脂多糖通过 Toll 样受体 4/NF-κB 介导的途径激活。
Infect Immun. 2018 Feb 20;86(3). doi: 10.1128/IAI.00642-17. Print 2018 Mar.
4
Lipopolysaccharide-binding alkylpolyamine DS-96 inhibits Chlamydia trachomatis infection by blocking attachment and entry.脂多糖结合烷基多胺DS-96通过阻断附着和进入来抑制沙眼衣原体感染。
Antimicrob Agents Chemother. 2014 Jun;58(6):3245-54. doi: 10.1128/AAC.02391-14. Epub 2014 Mar 24.
5
The role of lipooligosaccharide phosphorylcholine in colonization and pathogenesis of Histophilus somni in cattle.脂寡糖磷酸胆碱在牛嗜血杆菌定植和发病机制中的作用。
Vet Res. 2012 Jun 7;43(1):49. doi: 10.1186/1297-9716-43-49.
6
Global effects of catecholamines on Actinobacillus pleuropneumoniae gene expression.儿茶酚胺对胸膜肺炎放线杆菌基因表达的全球影响。
PLoS One. 2012;7(2):e31121. doi: 10.1371/journal.pone.0031121. Epub 2012 Feb 8.
7
Comparative genomic characterization of Actinobacillus pleuropneumoniae.胸膜肺炎放线杆菌的比较基因组特征分析。
J Bacteriol. 2010 Nov;192(21):5625-36. doi: 10.1128/JB.00535-10. Epub 2010 Aug 27.
8
Virulence factors of Actinobacillus pleuropneumoniae involved in colonization, persistence and induction of lesions in its porcine host.胸膜肺炎放线杆菌在猪体内定植、持续存在和诱导病变涉及的毒力因子。
Vet Res. 2010 Sep-Oct;41(5):65. doi: 10.1051/vetres/2010037. Epub 2010 Jun 15.
9
Host-pathogen interactions of Actinobacillus pleuropneumoniae with porcine lung and tracheal epithelial cells.胸膜肺炎放线杆菌与猪肺及气管上皮细胞的宿主-病原体相互作用
Infect Immun. 2009 Apr;77(4):1426-41. doi: 10.1128/IAI.00297-08. Epub 2009 Jan 12.
10
The major surface-associated saccharides of Klebsiella pneumoniae contribute to host cell association.肺炎克雷伯菌主要的表面相关糖类有助于其与宿主细胞结合。
PLoS One. 2008;3(11):e3817. doi: 10.1371/journal.pone.0003817. Epub 2008 Nov 26.
J Clin Microbiol. 1983 Dec;18(6):1351-4. doi: 10.1128/jcm.18.6.1351-1354.1983.
4
Porcine haemophili and actinobacilli: characterization by means of API test strips and possible taxonomic implications.
Can J Microbiol. 1984 Oct;30(10):1229-38. doi: 10.1139/m84-195.
5
Adherence of Haemophilus influenzae to monkey respiratory tissue in organ culture.流感嗜血杆菌在器官培养中对猴呼吸道组织的黏附作用。
J Gen Microbiol. 1984 Jun;130(6):1437-47. doi: 10.1099/00221287-130-6-1437.
6
Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains.从光滑型和粗糙型铜绿假单胞菌及鼠伤寒沙门氏菌菌株中分离细菌脂多糖的方法。
J Bacteriol. 1983 Aug;155(2):831-8. doi: 10.1128/jb.155.2.831-838.1983.
7
Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels.银染聚丙烯酰胺凝胶中沙门氏菌脂多糖化学型之间的形态异质性。
J Bacteriol. 1983 Apr;154(1):269-77. doi: 10.1128/jb.154.1.269-277.1983.
8
A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中脂多糖的灵敏银染法。
Anal Biochem. 1982 Jan 1;119(1):115-9. doi: 10.1016/0003-2697(82)90673-x.
9
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
10
Antibiotic susceptibility testing by a standardized single disk method.采用标准化单纸片法进行抗生素敏感性试验。
Am J Clin Pathol. 1966 Apr;45(4):493-6.