Division of Animal Infectious Disease, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
J Bacteriol. 2010 Nov;192(21):5625-36. doi: 10.1128/JB.00535-10. Epub 2010 Aug 27.
The Gram-negative bacterium Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumoniae, a lethal respiratory infectious disease causing great economic losses in the swine industry worldwide. In order to better interpret the genetic background of serotypic diversity, nine genomes of A. pleuropneumoniae reference strains of serovars 1, 2, 4, 6, 9, 10, 11, 12, and 13 were sequenced by using rapid high-throughput approach. Based on 12 genomes of corresponding serovar reference strains including three publicly available complete genomes (serovars 3, 5b, and 7) of this bacterium, we performed a comprehensive analysis of comparative genomics and first reported a global genomic characterization for this pathogen. Clustering of 26,012 predicted protein-coding genes showed that the pan genome of A. pleuropneumoniae consists of 3,303 gene clusters, which contain 1,709 core genome genes, 822 distributed genes, and 772 strain-specific genes. The genome components involved in the biogenesis of capsular polysaccharide and lipopolysaccharide O antigen relative to serovar diversity were compared, and their genetic diversity was depicted. Our findings shed more light on genomic features associated with serovar diversity of A. pleuropneumoniae and provide broader insight into both pathogenesis research and clinical/epidemiological application against the severe disease caused by this swine pathogen.
革氏阴性细菌胸膜肺炎放线杆菌是猪传染性胸膜肺炎的病原体，这种致命的呼吸道传染病在全球范围内给养猪业造成了巨大的经济损失。为了更好地解释血清型多样性的遗传背景，我们采用快速高通量方法对血清型 1、2、4、6、9、10、11、12 和 13 的 9 个胸膜肺炎放线杆菌参考菌株进行了测序。基于包括该细菌的三个公开的全基因组（血清型 3、5b 和 7）在内的 12 个相应血清型参考菌株的基因组，我们进行了全面的比较基因组学分析，并首次对该病原体进行了全球基因组特征描述。26012 个预测蛋白编码基因的聚类表明，胸膜肺炎放线杆菌的泛基因组由 3303 个基因簇组成，其中包含 1709 个核心基因组基因、822 个分布基因和 772 个菌株特异性基因。比较了与血清型多样性相关的荚膜多糖和脂多糖 O 抗原生物合成相关的基因组成分，并描绘了它们的遗传多样性。我们的研究结果更深入地了解了与胸膜肺炎放线杆菌血清型多样性相关的基因组特征，并为该猪病原体引起的严重疾病的发病机制研究和临床/流行病学应用提供了更广泛的认识。
