Li Kezhen, Jin Xin, Fang Yong, Wang Changyu, Gong Mei, Chen Pingbo, Liu Jia, Deng Dongrui, Ai Jihui
Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2012 Feb;32(1):97-102. doi: 10.1007/s11596-012-0017-7. Epub 2012 Jan 27.
The prevalence of human papilloma virus (HPV)-16 in patients with cervical cancer, the physical status of HPV-16 in patients with cervical lesions, and the role of HPV-16 integration in cervical carcinogenesis were investigated. HPV genotyping was performed by using PCR approach with the primer GP5+/GP6+ and type-specific primer on biopsy specimens taken operatively from 198 women. Multiple PCR was done to detect physical status of HPV-16 in a series of cervical liquid-based cytology samples and biopsy specimens obtained from different cervical lesions with HPV-16 infection, including 112 specimens with cervical cancer, 151 specimens with CIN I, 246 specimens with CIN and 120 specimens with CINIII. The results showed that there were 112 cervical cancer samples (56.57% of total cervical cancer patients) with HPV-16 infection. The frequency of HPV-16 pure integration was 65.18% (73/112), 56.57% (47/120), 23.58% (58/246) and 7.95% (12/151) in cervical cancer, CINIII, CINII and CINI patients respectively. In situ hybridization was performed on some paraffin-embedded sections of CINII, CINIII and cervical cancer to verify the physical status of HPV-16 infection. Significant difference was observed between cervical cancer and CIN I, CINII, CINIII in the frequency of HPV-16 integration (P<0.01). It is suggested that HPV-16 is the most prevalent type and is associated with cervical cancer. In the case of HPV-16 infection there are close associations between the severity of cervical lesions and the frequency of HPV-16 integration. The application of testing HPV genotyping and physical status based on detection of HC-II HPV DNA would be in favor of predicting the prognosis of cervical precancerosis and enhancing the screening accuracy of cervical cancer.
研究了宫颈癌患者中人乳头瘤病毒（HPV）-16的流行情况、宫颈病变患者中HPV-16的物理状态以及HPV-16整合在宫颈癌发生中的作用。采用PCR方法，使用引物GP5+/GP6+和型特异性引物，对198名女性手术切除的活检标本进行HPV基因分型。对一系列HPV-16感染的不同宫颈病变的宫颈液基细胞学样本和活检标本进行多重PCR，以检测HPV-16的物理状态，包括112例宫颈癌标本、151例CIN I标本、246例CIN II标本和120例CIN III标本。结果显示，112例宫颈癌样本（占宫颈癌患者总数的56.57%）感染了HPV-16。HPV-16纯整合频率在宫颈癌、CIN III、CIN II和CIN I患者中分别为65.18%（73/112）、56.57%（47/120）、23.58%（58/246）和7.95%（12/151）。对部分CIN II、CIN III和宫颈癌的石蜡包埋切片进行原位杂交，以验证HPV-16感染的物理状态。在HPV-16整合频率方面，宫颈癌与CIN I、CIN II、CIN III之间存在显著差异（P<0.01）。提示HPV-16是最常见的类型，与宫颈癌相关。在HPV-16感染的情况下，宫颈病变的严重程度与HPV-16整合频率密切相关。基于HC-II HPV DNA检测进行HPV基因分型和物理状态检测，有利于预测宫颈上皮内瘤变的预后，提高宫颈癌的筛查准确性。
