Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Sep Sci. 2012 Feb;35(4):608-15. doi: 10.1002/jssc.201100894.
Herein, a high-performance liquid chromatography-diode array detection method has been developed for the simultaneous determination of 15 phenolic antioxidants: flavan-3-ols, (-)-epigallocatechin, (+)-catechin, (-)-epigallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-gallocatechin, a phenolic acid (gallic acid), a hydroxycinnamic acid (chlorogenic acid), flavones (apigenin), flavonols (kaempferol, quercetin, and myricetin), and purine alkaloids (caffeine theophylline, theobromine) in different herb extracts, tea, and coffee varieties. The developed method was validated and successfully applied in order to determine the polyphenolic content to estimate the antioxidant activity of the Sideritis species commonly known as Greek mountain tea. To the best of our knowledge, this is the first report on the quantitative determination of catechins and other polyphenols in Greek mountain tea. Acidic hydrolysis was necessary for the simultaneous determination of the aglycones of the target analytes. According to our results, chlorogenic acid, myricetin, apigenin, catechin, and epicatechin gallate are found in the Sideritis species.
本文建立了一种高效液相色谱-二极管阵列检测法，用于同时测定 15 种酚类抗氧化剂：黄烷-3-醇、（-）-表没食子儿茶素、（+）-儿茶素、（-）-表没食子儿茶素没食子酸酯、（-）-表儿茶素、（-）-表儿茶素没食子酸酯、（-）-儿茶素、一种酚酸（没食子酸）、一种羟基肉桂酸（绿原酸）、类黄酮（芹菜素）、类黄酮醇（山奈酚、槲皮素和杨梅素）以及嘌呤生物碱（咖啡因、茶碱和可可碱）在不同草药提取物、茶和咖啡品种中的含量。该方法经过验证后成功地应用于测定多酚含量，以评估通常被称为希腊高山茶的缬草属植物的抗氧化活性。据我们所知，这是首次报道定量测定希腊高山茶中的儿茶素和其他多酚。为了同时测定目标分析物的糖苷配基，需要进行酸水解。根据我们的结果，在缬草属植物中发现了绿原酸、杨梅素、芹菜素、儿茶素和表儿茶素没食子酸酯。
