Lytra Krystalia, Tomou Ekaterina-Michaela, Chrysargyris Antonios, Drouza Chryssoula, Skaltsa Helen, Tzortzakis Nikolaos
Department of Pharmacognosy & Chemistry of Natural Products, School of Pharmacy, National & Kapodistrian University of Athens, Athens, Greece.
Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, Lemesos, Cyprus.
Front Pharmacol. 2020 May 12;11:650. doi: 10.3389/fphar.2020.00650. eCollection 2020.
species are recognized as important medicinal plants and their commercial demand is continuously on the rise both in the European and in the global market. Consequently, the cultivation of species has been occurred to successfully meet the need for mass production of high-quality plant material. The present study was undertaken in order to investigate the chemical composition of cultivated . Infusions of flowers and leaves were prepared separately, according to the European Medicine Agency (EMA) monograph. The infusion of the flowers revealed the presence of four flavones, isoscutellarein-7-O-[6'″-O-acetyl-β-D-allopyranosyl-(1→2)-β-D-glucopyranoside, its 4'-O-methyl-derivative, 4'-O-methyl-hypolaetin-7-O-[6'″-O-acetyl-β-D-allopyranosyl-(1→2)-β-D-glucopyranoside, and isoscutellarein-7-O-[6'″-O-acetyl-β-D-allopyranosyl-(1→2)]-6″-O-acetyl-β-D-glucopyranoside; four phenylethanoid glucosides, acteoside, leucosceptoside A, lamalboside, and leonoside A; one iridoid, melittoside, and one phenolic acid, chlorogenic acid, while the infusion of the leaves of the same population afforded the same first two flavones; five phenylethanoid glucosides, acteoside, leucosceptoside A, lavandulifolioside, leonoside A, and lamalboside; melittoside and chlorogenic acid. The structural elucidation of the isolated compounds was undertaken by high-field NMR spectroscopy. Moreover, the essential oils of the flowers and leaves were studied by GC-MS, separately. In addition, the mineral, bioactive compounds, protein and carbohydrate contents were evaluated for both plant materials.
该物种被公认为重要的药用植物,其在欧洲和全球市场的商业需求持续上升。因此,已经开展了该物种的种植以成功满足大规模生产高质量植物材料的需求。本研究旨在调查人工种植的该物种的化学成分。根据欧洲药品管理局(EMA)的专论,分别制备了花和叶的浸剂。花的浸剂中发现了四种黄酮,即异槲皮素 - 7 - O - [6'″ - O - 乙酰基 - β - D - 阿洛吡喃糖基 - (1→2) - β - D - 吡喃葡萄糖苷]、其4'-O - 甲基衍生物、4'-O - 甲基 - 异槲皮素 - 7 - O - [6'″ - O - 乙酰基 - β - D - 阿洛吡喃糖基 - (1→2) - β - D - 吡喃葡萄糖苷]和异槲皮素 - 7 - O - [6'″ - O - 乙酰基 - β - D - 阿洛吡喃糖基 - (1→2)] - 6″ - O - 乙酰基 - β - D - 吡喃葡萄糖苷;四种苯乙醇苷,即毛蕊花糖苷、白花败酱苷A、拉马波苷和狮尾草苷A;一种环烯醚萜苷,即蜜茱萸苷,以及一种酚酸,即绿原酸,而同一群体叶片的浸剂中含有相同的前两种黄酮;五种苯乙醇苷,即毛蕊花糖苷、白花败酱苷A、薰衣草叶苷、狮尾草苷A和拉马波苷;蜜茱萸苷和绿原酸。通过高场核磁共振光谱对分离得到的化合物进行了结构解析。此外,分别通过气相色谱 - 质谱联用仪(GC - MS)对花和叶的精油进行了研究。另外,对两种植物材料的矿物质、生物活性化合物、蛋白质和碳水化合物含量进行了评估。