Rottini G, Dobrina A, Forgiarini O, Nardon E, Amirante G A, Patriarca P
Institute of General Pathology, University of Trieste, Italy.
Infect Immun. 1990 Nov;58(11):3751-8. doi: 10.1128/iai.58.11.3751-3758.1990.
Generation and release into the culture medium of a cytolytic toxin by Gardnerella vaginalis has been demonstrated. Addition of starch and of the nonionic detergent Tween 80 to the culture medium was essential to recover cytolytic activity. A protein with an apparent molecular mass of 61 to 63 kDa was purified from the culture supernatants showing lytic activity towards erythrocytes and nucleated cells, such as human endothelial cells and human neutrophils. The protein had marked selectivity for human erythrocytes, while erythrocytes from other species were not lysed or were lysed at much higher concentrations of the protein than those needed for human erythrocytes. The cytolytic activity was remarkably unstable in polar media, but was stabilized by nonionic detergents, by binding, or by insertion into the target cell membrane, suggesting its amphiphilic nature.
阴道加德纳菌可产生一种溶细胞毒素并释放到培养基中，这一点已得到证实。向培养基中添加淀粉和非离子去污剂吐温80对于恢复溶细胞活性至关重要。从培养上清液中纯化出一种表观分子量为61至63 kDa的蛋白质，该蛋白质对红细胞以及有核细胞（如人内皮细胞和人中性粒细胞）具有裂解活性。该蛋白质对人红细胞具有明显的选择性，而来自其他物种的红细胞不会被裂解，或者需要比人红细胞所需浓度高得多的该蛋白质才能被裂解。溶细胞活性在极性介质中非常不稳定，但可通过非离子去污剂、结合或插入靶细胞膜而得到稳定，这表明其具有两亲性。
