Berka R M, Gray G L, Vasil M L
Infect Immun. 1981 Dec;34(3):1071-4. doi: 10.1128/iai.34.3.1071-1074.1981.
The chromogenic substrate p-nitrophenylphosphorylcholine was used to measure phospholipase C (heat-labile hemolysin) production by clinical strains of Pseudomonas aeruginosa. Analysis of strains from various types of infection showed that phospholipase C production by urinary tract isolates was significantly greater than that by lung, blood, or other isolates (e.g., wounds, etc.). The above method was also found to be effective for the isolation of several classes of phospholipase C mutants.
采用生色底物对硝基苯磷酰胆碱来检测铜绿假单胞菌临床菌株产生磷脂酶C（不耐热溶血素）的情况。对来自各种感染类型的菌株分析表明，泌尿道分离株产生的磷脂酶C显著多于肺部、血液或其他分离株（如伤口等部位的分离株）。还发现上述方法对于分离几类磷脂酶C突变体有效。
