Indian J Microbiol. 2011 Jan;51(1):8-13. doi: 10.1007/s12088-011-0068-7. Epub 2011 Jan 26.
A combination of cultivation-based methods with a molecular biological approach was employed to investigate whether bacteria with identical 16S rRNA gene sequences can represent distinct eco- and genotypes. A set of eight bacterial strains wherein three were Pseudomonas putida and rest were Acinetobacter calcoaceticus, were isolated from casing soils community by conventional plating. These strains had identical 16S rRNA gene sequences and represented the dominant phylotype in the plateable fraction. Each strain utilized a specific combination of 154 carbon substrates, and the niche overlap indices were low, suggesting that each strain occupied a different ecological niche. Our results have implications for assessment of the diversity and biogeography of bacteria and increase the perception of natural diversity beyond the level of 16S rRNA gene sequences. It is worthwhile approach to explore prokaryotic diversity in different ecological niches.
采用基于培养的方法与分子生物学方法相结合，研究了具有相同 16S rRNA 基因序列的细菌是否可以代表不同的生态和基因型。从外壳土壤群落中通过常规平板分离出 8 株细菌，其中 3 株为假单胞菌，其余为醋酸钙不动杆菌。这些菌株具有相同的 16S rRNA 基因序列，并且是可平板计数部分的主要优势型。每个菌株利用特定的 154 种碳底物组合，生态位重叠指数较低，表明每个菌株占据不同的生态位。我们的研究结果对于评估细菌的多样性和生物地理学具有重要意义，并提高了对细菌多样性的认识，超越了 16S rRNA 基因序列的水平。探索不同生态位中的原核生物多样性是一种值得探索的方法。
