Doillon C J, Cameron K
St-François d'Assise's Hospital, Québec, Canada.
Int J Artif Organs. 1990 Aug;13(8):517-20.
We are proposing two modified assays for biocompatibility testing which analyze the effects of cytotoxic substances leached from a biomaterial in cell culture. The biocompatibility of two vascular prostheses made of polytetrafluoroethylene was analyzed using the modified assays. One test, the "fluid medium assay" was modified by using small pieces of graft glued to a screening lid, thereby reducing the possibility of mechanical injury to cultured cells by free fragments of the tested biomaterial. Another test, the "cell inhibition assay" was modified in that the biomaterial to be tested was ground into small pieces while at very low temperature. The measure of cell toxicity used was the effect on DNA replication. Our results suggest that these modified assay methods can be used to evaluate the biocompatibility of biomaterials.
我们提出了两种用于生物相容性测试的改良试验方法，这些方法用于分析细胞培养中从生物材料沥出的细胞毒性物质的影响。使用改良试验方法分析了两种由聚四氟乙烯制成的血管假体的生物相容性。一种试验，即“液体培养基试验”，通过将小块移植物粘在筛选盖上进行了改良，从而降低了受试生物材料的游离碎片对培养细胞造成机械损伤的可能性。另一种试验，即“细胞抑制试验”，其改良之处在于将受试生物材料在非常低的温度下研磨成小块。所使用的细胞毒性测量方法是对DNA复制的影响。我们的结果表明，这些改良的试验方法可用于评估生物材料的生物相容性。
