Mechtersheimer G, Staudter M, Majdic O, Dörken B, Moldenhauer G, Möller P
Institute of Pathology, University of Heidelberg, FRG.
Int J Cancer. 1990 Nov 15;46(5):813-23. doi: 10.1002/ijc.2910460512.
Non-neoplastic mesenchymal cells, along with 33 benign and 87 malignant soft-tissue tumors (STT) were examined for expression of HLA-A,B,C, beta 2-microglobulin (beta 2m), HLA-DR, -DP, and -DQ molecules and the HLA-D associated invariant chain (Ii). Serial frozen sections were immunostained using monoclonal antibodies (MAbs) to monomorphic framework determinants of HLA sublocus products, beta 2m and Ii, and to CD53, a recently defined broadly distributed pan-leucocyte molecule. Compared with the normal state, an induction/neo-expression of HLA-A,B,C/beta 2m was found in a considerable number of tumors of muscle, peripheral nerve, cartilage-forming, adipose, and vascular tissues. Conversely, some tumors of fibrous origin and of autonomic ganglia showed an abnormal abrogation/loss of HLA-A,B,C/beta 2m with respect to their cells of origin. Small, round tumor cells present in various types of STT exhibited a heterogenous pattern of expression of these molecules with a preponderance of HLA-A, B,C/beta 2m-negativity. HLA-D/Ii determinants were rarely detectable in STT. Besides their expression in some fibrohistiocytic tumors, they were only occasionally found in tumors of smooth-muscle, peripheral-nerve and vascular origin as well as in one clear-cell sarcoma. In all tumors but one, there was no microtopographic association between HLA-D/Ii-positive tumor cells and inflammatory cells. CD53 allowed discrimination between dendritic interstitial cells (DIC) and neoplastic cells and additionally revealed that, in contrast to other solid tumors, STT are generally characterized by an extreme scarcity of lymphohistiocytic infiltrates. Our data indicate that, aside from very rare exceptions, aberrant induction or abrogation of MHC molecules in STT occurs in the absence of lymphohistiocytic stromal infiltrates, suggesting that these alterations might not be a consequence of local cytokine effects.
对非肿瘤性间充质细胞以及33例良性和87例恶性软组织肿瘤（STT）进行检测，观察其HLA - A、B、C、β2 - 微球蛋白（β2m）、HLA - DR、- DP和 - DQ分子以及HLA - D相关恒定链（Ii）的表达情况。使用针对HLA亚位点产物、β2m和Ii的单态性框架决定簇以及CD53（一种最近定义的广泛分布的全白细胞分子）的单克隆抗体（MAb）对连续冰冻切片进行免疫染色。与正常状态相比，在相当数量的肌肉、外周神经、软骨形成、脂肪和血管组织肿瘤中发现了HLA - A、B、C/β2m的诱导/新表达。相反，一些纤维起源的肿瘤和自主神经节肿瘤相对于其起源细胞显示出HLA - A、B、C/β2m的异常缺失/丧失。存在于各种类型STT中的小圆形肿瘤细胞表现出这些分子表达的异质性模式，以HLA - A、B、C/β2m阴性为主。HLA - D/Ii决定簇在STT中很少能检测到。除了在一些纤维组织细胞瘤中表达外，它们仅偶尔在平滑肌、外周神经和血管起源的肿瘤以及一例透明细胞肉瘤中发现。在除一例之外的所有肿瘤中，HLA - D/Ii阳性肿瘤细胞与炎症细胞之间不存在微观地形学关联。CD53能够区分树突状间质细胞（DIC）和肿瘤细胞，此外还显示，与其他实体瘤不同，STT的一般特征是淋巴细胞组织细胞浸润极少。我们的数据表明，除了极少数例外情况，STT中MHC分子的异常诱导或缺失发生在没有淋巴细胞组织细胞间质浸润的情况下，这表明这些改变可能不是局部细胞因子作用的结果。
