Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, QC H3A 2K6 Canada.
J Am Chem Soc. 2012 Feb 15;134(6):2888-91. doi: 10.1021/ja2107492. Epub 2012 Feb 2.
DNA nanotubes hold promise as scaffolds for protein organization, as templates of nanowires and photonic systems, and as drug delivery vehicles. We present a new DNA-economic strategy for the construction of DNA nanotubes with a backbone produced by rolling circle amplification (RCA), which results in increased stability and templated length. These nanotubes are more resistant to nuclease degradation, capable of entering human cervical cancer (HeLa) cells with significantly increased uptake over double-stranded DNA, and are amenable to encapsulation and release behavior. As such, they represent a potentially unique platform for the development of cell probes, drug delivery, and imaging tools.
DNA 纳米管有望成为蛋白质组织、纳米线和光子系统模板以及药物输送载体的支架。我们提出了一种新的 DNA 经济策略，用于构建由滚环扩增（RCA）产生的骨架的 DNA 纳米管，这导致了稳定性和模板长度的增加。这些纳米管对核酸酶的降解更具抵抗力，能够进入人宫颈癌（HeLa）细胞，与双链 DNA 相比，摄取量显著增加，并且易于封装和释放。因此，它们为开发细胞探针、药物输送和成像工具提供了一个潜在的独特平台。
