Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
Adv Sci (Weinh). 2022 Feb;9(4):e2104084. doi: 10.1002/advs.202104084. Epub 2021 Dec 16.
Herein, a programmable dual-catalyst hairpin assembly (DCHA) for realizing the synchronous recycle of two catalysts is developed, displaying high reaction rate and outstanding conversion efficiency beyond traditional nucleic acid signal amplifications (NASA). Once catalyst I interacts with the catalyst II, the DCHA can be triggered to realize the simultaneous recycle of catalysts I and II to keep the highly concentrated intermediate product duplex I-II instead of the steadily decreased one in typical NASA, which can accomplish in about only 16 min and achieves the outstanding conversion efficiency up to 4.54 × 10 , easily conquering the main predicaments of NASA: time-consuming and low-efficiency. As a proof of the concept, the proposed DCHA as a high-speed and hyper-efficiency DNA signal magnifier is successfully applied in the rapid and ultrasensitive detection of miRNA-21 in cancer cell lysates, which exploits the new generation of universal strategy for the applications in biosensing assay, clinic diagnose, and DNA nanobiotechnology.
本文开发了一种可编程双催化剂发夹组装（DCHA），用于实现两种催化剂的同步回收，显示出高于传统核酸信号放大（NASA）的高反应速率和出色的转化效率。一旦催化剂 I 与催化剂 II 相互作用，DCHA 就可以被触发，实现催化剂 I 和 II 的同时回收，以保持高浓度的中间体双链 I-II，而不是典型 NASA 中稳定减少的中间体，这可以在大约 16 分钟内完成，并实现高达 4.54×10 的出色转化效率，轻松克服 NASA 的主要困境：耗时和低效率。作为概念验证，所提出的 DCHA 作为高速和超高效 DNA 信号放大器，成功应用于癌细胞裂解物中 miRNA-21 的快速和超灵敏检测，利用新一代通用策略在生物传感分析、临床诊断和 DNA 纳米生物技术中的应用。
