Advanced Analysis Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryangri, Seoul 136-791, Republic of Korea.
Forensic Sci Int. 2012 Jul 10;220(1-3):e23-8. doi: 10.1016/j.forsciint.2011.12.011. Epub 2012 Jan 28.
Some cosmetic products manufactured in Korea for the treatment of eczema, seborrhea and psoriasis have been suspected to contain anti-inflammatory corticosteroids such as prednisolone, hydrocortisone, betamethasone, dexamethasone and triamcinolone acetonide without these ingredients being indicated on the label. Due to their severe side effects such as permenent skin atopy, these corticosteroids have to be monitored in cosmetic products from a forensic point of view. Many cosmetic product samples (N=65) have been collected from both local and online markets in Korea. The corticosteroid content of these samples was analyzed by LC-MS/MS with diagnostic ions (m/z). Linearity was studied with 0.1-10 μg/mL range in all corticosteroids. Good correlation coefficients (r(2)≥0.997) were found and the limits of quantification were 4.68-7.97 ng/mL for each of the corticosteroids. At three different concentrations spanning the linear dynamic ranges, mean recoveries were 97.2-113.5%and precisions (RSD) for intra-day and inter-day analysis were less than 8.9%. Also, accuracy (Bias %) was less than 11.8%. The results showed that between 0.76-0.94 μg/g levels of prednisolone were detected in four cosmetic products and triamcinolone acetonidewas detected with a concentration in the range of 11.5-272 μg/g in nine samples. This fact reveals that some manufacturers have arbitrarily added these corticosteroids in their cosmetic products without indicating them on the label. Thus, these cosmetic products have to be monitored and if proven illegal preparations removed from the market.
一些在韩国制造的用于治疗湿疹、脂溢性皮炎和银屑病的化妆品被怀疑含有倍他米松、氢化可的松、倍氯米松、地塞米松和曲安奈德等抗炎皮质类固醇，但这些成分并未在标签上注明。由于这些皮质类固醇具有永久性皮肤过敏等严重副作用，因此必须从法医学角度对化妆品中的这些物质进行监测。从当地和网上市场共采集了 65 个化妆品样本。采用 LC-MS/MS 分析这些样本中的皮质类固醇含量，用诊断离子（m/z）进行分析。所有皮质类固醇的线性范围均为 0.1-10 μg/mL。均发现良好的相关性系数（r(2)≥0.997），每个皮质类固醇的定量限为 4.68-7.97ng/mL。在跨越线性动态范围的三个不同浓度下，平均回收率为 97.2-113.5%，日内和日间分析的精密度（RSD）小于 8.9%。此外，准确度（偏差%）小于 11.8%。结果表明，在四种化妆品中检测到 0.76-0.94μg/g 水平的泼尼松龙，在九种样本中检测到曲安奈德的浓度范围为 11.5-272μg/g。这一事实表明，一些制造商在其化妆品中任意添加了这些皮质类固醇，而未在标签上注明。因此，必须对这些化妆品进行监测，如果发现是非法制剂，应从市场上予以清除。
