College of Science, Beijing Technology and Business University, Beijing, 102488, China.
Anal Bioanal Chem. 2019 Sep;411(22):5703-5710. doi: 10.1007/s00216-019-01948-2. Epub 2019 Jul 24.
Dexamethasone (DE) is a synthetic glucocorticoid that is frequently added to cosmetic products for its good short-term effects, especially in facial masks, but long-term use is hazardous to the health. The abuse of DE in whitening and acne cosmetic products is currently a serious problem in China. It is necessary to establish a rapid method of detecting illegal DE addition in cosmetics. In the present study, a monoclonal antibody (mAb) against DE, 2D5-3D12, was developed that displayed cross-reactivities of 124.5%, 38.8%, 6.7%, 0.9%, 1.1%, 1.82%, and 2.39% with prednisolone, betamethasone, prednisone, beclomethasone, hydrocortisone, triamcinolone, and flumetasone, respectively. A colloidal gold-based lateral flow immunographic assay based on mAb 2D5-3D12 was established and used to determine the DE contents of commercial facial masks. The indicator range of the immunographic assay for DE was 100-200 ng/mL, and the results were consistent with those afforded by LC-MS. This novel method provides the advantages of simple sample treatment, a user-friendly procedure, and rapid detection. Graphical abstract.
地塞米松（DE）是一种合成糖皮质激素，因其短期效果好而经常添加到化妆品中，尤其是在面膜中，但长期使用对健康有害。在中国，DE 在美白和痤疮化妆品中的滥用目前是一个严重的问题。有必要建立一种快速检测化妆品中非法添加 DE 的方法。在本研究中，开发了一种针对 DE 的单克隆抗体（mAb），2D5-3D12，其与泼尼松龙、倍他米松、泼尼松、倍氯米松、氢化可的松、曲安奈德和氟米龙的交叉反应性分别为 124.5%、38.8%、6.7%、0.9%、1.1%、1.82%和 2.39%。建立了基于 mAb 2D5-3D12 的胶体金侧向流动免疫层析法，并用于测定市售面膜中的 DE 含量。免疫层析法测定 DE 的指标范围为 100-200ng/mL，结果与 LC-MS 一致。该新方法具有样品处理简单、操作方便、快速检测等优点。
