Tehran University of Medical Sciences, The Cancer Research Center, Tehran, Iran.
Eur J Intern Med. 2012 Mar;23(2):e59-62. doi: 10.1016/j.ejim.2011.06.011. Epub 2011 Nov 12.
Considering the different results obtained regarding the association of HPV with the development of esophageal squamous cell carcinoma (ESCC) in different populations, we aimed to determine the frequency of HPV infection and its subtypes in ESCC in Iranian patients.
A total of 100 paraffin-embedded tissue samples of ESCC diagnosed during 1991 and 2005 in the Institute of Cancer affiliated to Tehran University of Medical Sciences were selected. Seven out of 100 samples were excluded due to low quality of extracted DNA from paraffin-embedded specimens. Thus, 93 samples were included for HPV genotyping.
All samples were examined using SPF10 primers for HPV detection. HPV DNA was positive in 8 out of 93 (8.6%) ESCC specimens. Using INNO-LiPA genotyping system we detected the genotypes of 5 out of 8 HPV-positive samples. Both HPV types 16 and 6 were detected in 3 specimens; one sample was positive for HPV type 18 and 2 samples were co-infected with two HPV types. There were no statistically significant differences between HPV-positive and HPV-negative cases with regard to clinical and pathologic findings. Three samples were positive for SPF10 indicating HPV infection; however, the exact HPV type could not be clarified using INNO-LiPA genotyping .
In conclusion, the present study showed that a small proportion of ESCC specimens from Iran harbor HPV16, 18 genome using a highly sensitive method. As different rates have been reported from Iran, a more widespread study with more precise definition of geographical differences could delineate the potential involvement of HPV in the development of ESCC in Iranian population.
鉴于 HPV 与食管鳞状细胞癌（ESCC）发展之间的关联在不同人群中得到的结果不同，我们旨在确定 HPV 感染及其在伊朗患者 ESCC 中的亚型的频率。
选择了 1991 年至 2005 年期间在德黑兰医科大学癌症研究所诊断的 100 例 ESCC 石蜡包埋组织样本。由于从石蜡包埋标本中提取 DNA 的质量低，其中 7 例被排除在外。因此，对 93 例样本进行 HPV 基因分型。
所有样本均使用 SPF10 引物检测 HPV。在 93 例 ESCC 标本中，有 8 例（8.6%）HPV DNA 阳性。使用 INNO-LiPA 基因分型系统，我们检测到 8 例 HPV 阳性样本中的 5 种基因型。3 例标本同时检测到 HPV 型 16 和 6；1 例标本检测到 HPV 型 18，2 例标本同时感染了两种 HPV 型。HPV 阳性和 HPV 阴性病例在临床和病理发现方面无统计学差异。3 例标本对 SPF10 呈阳性，表明 HPV 感染；然而，使用 INNO-LiPA 基因分型无法明确确切的 HPV 类型。
总之，本研究表明，使用高度敏感的方法，一小部分来自伊朗的 ESCC 标本携带 HPV16、18 基因组。由于伊朗报道的比率不同，更广泛的研究和更精确的地理位置差异定义可以描绘 HPV 在伊朗人群中发展 ESCC 的潜在作用。
