Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS), affiliated with Université Joseph Fourier and member of the Institut de Chimie Moléculaire de Grenoble, BP 53, F-38041 Grenoble Cedex 9, France.
J Colloid Interface Sci. 2012 Mar 15;370(1):58-66. doi: 10.1016/j.jcis.2011.12.063. Epub 2012 Jan 8.
Polycaprolactone (PCL) nanoparticles decorated with a mucoadhesive polysaccharide chitosan (CS) containing curcumin were developed aiming the buccal delivery of this drug. These nanoparticles were prepared by the nanoprecipitation method using different molar masses and concentrations of chitosan and concentrations of triblock surfactant poloxamer (PEO-PPO-PEO), in order to optimize the preparation conditions. Chitosan-coated nanoparticles showed positive surface charge and a mean particle radius ranging between 114 and 125 nm, confirming the decoration of the nanoparticles with the mucoadhesive polymer, through hydrogen bonds between ether and amino groups from PEO and CS, respectively. Dynamic Light Scattering (DLS) studies at different scattering angles and concentrations have shown that the nanoparticles are monodisperse (polydispersity indices were lower than 0.3). The nanoparticle systems were also examined with Nanoparticle Tracking Analysis (NTA), and the results were in good agreement with those obtained by DLS. Colloidal systems showed mean drug content about 460 μg/mL and encapsulation efficiency higher than 99%. Finally, when coated with chitosan, these nanoparticles show a great ability to interact with mucin indicating also their suitability for mucoadhesive applications.
聚己内酯（PCL）纳米粒子用含有姜黄素的黏附多糖壳聚糖（CS）进行修饰，旨在通过口腔给药。这些纳米粒子是通过纳米沉淀法制备的，使用了不同摩尔质量和浓度的壳聚糖和三嵌段表面活性剂泊洛沙姆（PEO-PPO-PEO），以优化制备条件。壳聚糖包覆的纳米粒子具有正表面电荷和 114 至 125nm 之间的平均粒径，通过 PEO 和 CS 的醚和氨基之间的氢键，证实了纳米粒子与黏附聚合物的修饰。在不同散射角和浓度下的动态光散射（DLS）研究表明，纳米粒子是单分散的（多分散指数低于 0.3）。还使用纳米颗粒跟踪分析（NTA）对纳米颗粒系统进行了检查，结果与 DLS 获得的结果非常吻合。胶体系统的平均药物含量约为 460μg/mL，包封效率高于 99%。最后，当用壳聚糖包覆时，这些纳米粒子显示出与粘蛋白相互作用的巨大能力，这也表明它们适合用于黏附应用。
