J Biomed Nanotechnol. 2014 May;10(5):787-94. doi: 10.1166/jbn.2014.1768.
Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) was used to investigate the mucoadhesive properties of nanoparticles decorated with low, medium and high molar mass chitosan (CS). Uncoated and chitosan-coated polycaprolactone (PCL) nanoparticles loaded with curcumin were prepared by nanoprecipitation method and characterized in terms of size, surface charge and drug content. The interactions between nanoparticles and mucin layer were monitored after the treatment of SAM-functionalized gold-coated quartz crystals with bovine submaxillary gland mucin (BSM). The results show that all investigated chitosan-coated nanoparticles adsorb onto the BSM layer, and the mass uptake was found to be independent of the chitosan molar mass. Uncoated nanoparticles showed, however, no affinity with BSM layer, confirming that the adsorption of colloidal systems occurs due to their decoration with chitosan. The adhesion is mainly attributed to electrostatic interactions between protonated amino groups of mucoadhesive chitosan and negatively charged groups of mucin. The results suggest that chitosan-coated nanoparticles are promising carriers for hydrophobic drugs delivery in the buccal mucosa.
采用石英晶体微天平（QCM-D）技术研究了低、中、高相对分子质量壳聚糖（CS）修饰纳米粒的黏附性能。采用纳米沉淀法制备了姜黄素载药的未涂层和壳聚糖涂层聚己内酯（PCL）纳米粒，并对其粒径、表面电荷和载药量进行了表征。用牛颌下腺黏蛋白（BSM）处理 SAM 功能化镀金石英晶体后，监测纳米粒与黏蛋白层之间的相互作用。结果表明，所有研究的壳聚糖涂层纳米粒都能吸附到 BSM 层上，且质量吸收与壳聚糖的相对分子质量无关。然而，未涂层的纳米粒与 BSM 层没有亲和力，这证实了胶体系统的吸附是由于其被壳聚糖修饰所致。黏附主要归因于带正电荷的黏附性壳聚糖的氨基基团与黏蛋白的负电荷基团之间的静电相互作用。结果表明，壳聚糖涂层纳米粒有望成为口腔黏膜中疏水性药物传递的载体。
