缺失 Hoc 和 Soc 衣壳蛋白会影响源自噬菌体 T4 的纳米颗粒的表面和细胞摄取特性。
Deletion of the Hoc and Soc capsid proteins affects the surface and cellular uptake properties of bacteriophage T4 derived nanoparticles.
机构信息
Center of for Bio/Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, United States.
出版信息
Biochem Biophys Res Commun. 2012 Feb 17;418(3):537-40. doi: 10.1016/j.bbrc.2012.01.061. Epub 2012 Jan 21.
Abstract
Recently the use of engineered viral scaffolds in biotechnology and medical applications has been increasing dramatically. T4 phage capsid derived nanoparticles (NPs) have potential advantages as sensors and in biotechnology. These applications require that the physical properties and cellular uptake of these NPs be understood. In this study we used a T4 deletion mutant to investigate the effects of removing both the Hoc and Soc proteins from the capsid surface on T4 tailless NPs. The surface charge, zeta potential, size, and cellular uptake efficiencies for both the T4 NP and T4ΔHocΔSoc NP mutant were measured and compared using dynamic light scattering and flow cytometry and significant differences were detected.
摘要
最近，工程病毒支架在生物技术和医学应用中的使用急剧增加。T4 噬菌体衣壳衍生的纳米颗粒（NPs）作为传感器和生物技术具有潜在的优势。这些应用要求了解这些 NPs 的物理性质和细胞摄取。在这项研究中，我们使用 T4 缺失突变体来研究从衣壳表面去除 Hoc 和 Soc 蛋白对 T4 无尾 NPs 的影响。使用动态光散射和流式细胞术测量并比较了 T4 NP 和 T4ΔHocΔSoc NP 突变体的表面电荷、zeta 电位、大小和细胞摄取效率，并检测到显著差异。
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