噬菌体 T4 纳米颗粒作为传感器应用中的材料：影响其在表面上的组织和组装的变量。

U.S. Naval Research Laboratory/4555 Overlook Ave., SW, Center for Biomolecular Science and Engineering, Washington, D.C. 20375, USA; E-Mail:

Sensors (Basel). 2009;9(8):6298-311. doi: 10.3390/s90806298. Epub 2009 Aug 12.

Bacteriophage T4 nanoparticles possess characteristics that make them ideal candidates as materials for sensors, particularly as sensor probes. Their surface can be modified, either through genetic engineering or direct chemical conjugation to display functional moieties such as antibodies or other proteins to recognize a specific target. However, in order for T4 nanoparticles to be utilized as a sensor probe, it is necessary to understand and control the variables that determine their assembly and organization on a surface. The aim of this work is to discuss some of variables that we have identified as influencing the behavior of T4 nanoparticles on surfaces. The effect of pH, ionic strength, substrate characteristics, nanoparticle concentration and charge was addressed qualitatively using atomic force microscopy (AFM).

T4 噬菌体纳米颗粒具有使其成为传感器材料的理想特性，尤其是作为传感器探针。它们的表面可以通过遗传工程或直接化学偶联进行修饰，以显示功能部分，如抗体或其他蛋白质，以识别特定的目标。然而，为了将 T4 噬菌体纳米颗粒用作传感器探针，有必要了解和控制决定它们在表面上组装和组织的变量。本工作的目的是讨论一些我们已经确定的影响 T4 噬菌体纳米颗粒在表面上行为的变量。使用原子力显微镜（AFM）定性地研究了 pH、离子强度、基底特性、纳米颗粒浓度和电荷的影响。