Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareli Road, Lucknow 226014, UP, India.
Reprod Biomed Online. 2012 Mar;24(3):342-51. doi: 10.1016/j.rbmo.2011.11.021. Epub 2011 Dec 10.
The aim of this study was to determine whether or not interleukin-1 alpha (IL-1a), interleukin-1 beta (IL-1b) and IL-1 receptor antagonist (IL-1RA) polymorphisms are associated with risk of unexplained recurrent pregnancy loss (RPL) among North Indian women. This retrospective case–control study examine 200 well-characterized RPL cases for IL-1 gene cluster variants, determined by restriction fragment length polymorphism-PCR. The observed allele, genotype and haplotype distributions were compared with those obtained from 300 ethnically matched negative controls. Invariant distribution of IL-1 gene cluster single-nucleotide polymorphisms was observed among RPL cases and controls. Meta-analysis of IL-1b _511, +3953 and IL-1RN 86-bp variable number tandem repeat from the reported literature and this study did not reveal any significant association with the risk of RPL. In conclusion, no significant difference between RPL and control groups was observed at the allele, genotype or haplotype levels when tested for association using the dominant, recessive and additive models of inheritance for IL-1 gene cluster variants. As far as is known, this is the first report from India pertaining to IL-1 gene cluster variants’ association with the risk of RPL from North India.
本研究旨在确定白细胞介素-1 阿尔法（IL-1a）、白细胞介素-1 贝塔（IL-1b）和白细胞介素-1 受体拮抗剂（IL-1RA）多态性是否与印度北部妇女不明原因复发性妊娠丢失（RPL）的风险相关。这项回顾性病例对照研究通过限制性片段长度多态性聚合酶链反应（PCR）检查了 200 例特征明确的 RPL 病例的 IL-1 基因簇变异。观察到的等位基因、基因型和单倍型分布与从 300 名种族匹配的阴性对照中获得的分布进行了比较。在 RPL 病例和对照组中观察到 IL-1 基因簇单核苷酸多态性的不变分布。对来自报道文献和本研究的 IL-1b _511、+3953 和 IL-1RN 86-bp 可变数串联重复的 IL-1 基因簇多态性进行荟萃分析，并未发现与 RPL 风险相关的任何显著关联。总之，在使用 IL-1 基因簇变异的显性、隐性和加性遗传模型对 RPL 和对照组进行关联测试时，在等位基因、基因型或单倍型水平上未观察到 RPL 组与对照组之间存在显著差异。据了解，这是印度第一份关于 IL-1 基因簇变异与印度北部 RPL 风险相关的报告。
