Department of Oral Medicine & Oral Surgery, Dental School, Jordan University of Science & Technology, Irbid, Jordan.
Arch Oral Biol. 2011 Mar;56(3):269-76. doi: 10.1016/j.archoralbio.2010.10.001. Epub 2010 Oct 29.
Interleukin-1 (IL-1) is a proinflammatory cytokine that is highly elevated in response to bacterial biofilms and is a potential risk factor for periodontal diseases. IL-1 gene polymorphisms have been associated with the IL-1 level. The aim of this study was to investigate if IL-1 gene cluster polymorphisms are associated with chronic (CP) and aggressive (AgP) periodontitis in a Jordanian population.
A total of 100 CP, 80 AgP patients and 80 controls were genotyped using PCR for IL-1RN-86-bp VNTR and PCR-RFLP for IL-1A-889, IL-1B-511, -35, +3953, and IL-1RN +8006, +9589, +11100 SNPs. The distribution of alleles and genotypes between groups was compared using χ(2) analysis. Estimation of haplotype frequencies was carried out using the EH programme.
The IL-1RN8006 SNP and the IL-1RN-VNTR were associated with CP but not with AgP. The C allele and TC genotype of IL-1RN8006 were increased in CP (P(corr)=0.002, 0.00026 respectively). The A1 allele and A1/A1 genotype of the IL1-RN-VNTR were significantly increased in CP (P(corr)=0.0007, <0.0001 respectively). The CA1 haplotype formed by both markers was present in 29 CP patients but not in any of the controls (P<0.0001). No significant differences were found in the distribution of allele and genotype frequencies of the other markers between CP and AgP cases and controls.
IL-1RN 8006 and IL-1RN VNTR were associated with CP but not AgP in a Jordanian population, whilst other investigated markers in IL-1A, IL-1B and IL-1RN were not associated with either CP or AgP.
白细胞介素-1（IL-1）是一种促炎细胞因子，对细菌生物膜的反应高度升高，是牙周病的潜在危险因素。IL-1 基因多态性与 IL-1 水平有关。本研究旨在探讨白细胞介素-1 基因簇多态性是否与约旦人群的慢性（CP）和侵袭性（AgP）牙周炎有关。
采用聚合酶链反应（PCR）对 100 例 CP、80 例 AgP 患者和 80 例对照组的 IL-1RN-86-bpVNTR 进行基因分型，采用 PCR-RFLP 对 IL-1A-889、IL-1B-511、-35、+3953 和 IL-1RN+8006、+9589、+11100SNP 进行基因分型。采用卡方检验比较组间等位基因和基因型的分布。采用 EH 程序估计单倍型频率。
IL-1RN8006SNP 和 IL-1RN-VNTR 与 CP 相关，但与 AgP 无关。CP 中 IL-1RN8006 的 C 等位基因和 TC 基因型增加（P（校正）=0.002，0.00026 分别）。IL-1-RN-VNTR 的 A1 等位基因和 A1/A1 基因型在 CP 中显著增加（P（校正）=0.0007，<0.0001 分别）。由两个标记形成的 CA1 单倍型存在于 29 例 CP 患者中，但不存在于任何对照组中（P<0.0001）。CP 和 AgP 病例与对照组之间，其他标记物的等位基因和基因型频率分布无显著差异。
在约旦人群中，IL-1RN8006 和 IL-1RNVNTR 与 CP 相关，但与 AgP 无关，而 IL-1A、IL-1B 和 IL-1RN 中其他研究的标记物与 CP 或 AgP 均无关。
