Balamurugan Kuppareddi, Duncan George
School of Criminal Justice, University of Southern Mississippi, 118 College Drive, Hattiesburg, MS 39406, United States.
Leg Med (Tokyo). 2012 Mar;14(2):105-9. doi: 10.1016/j.legalmed.2011.12.002. Epub 2012 Jan 28.
We have analyzed 17 Y-chromosomal STR loci in a population sample of 69 unrelated male individuals of the Rwanda-Hutu population from East Central Africa using an AmpFlSTR® Yfiler™ PCR amplification kit. A total of 62 unique haplotypes were identified among the 69 individuals studied. The haplotype diversity was found to be 0.9970 for this population. The gene diversity ranged from 0.1130 (DYS392) to 0.7722 (DYS385). Comparison of populations in this study with twenty-five other national and global populations using Principal Co-ordinate Analysis (PCA) and phylogenetic molecular analysis using a genetic distance matrix indicates a delineation of all the African populations from other unrelated populations. The results of population pair-wise Fst p values indicate statistically significant differentiation of the Rwandan population when compared with 25 other global populations including four African populations (p=0.0000). Analysis of Molecular Variance (AMOVA) of the Rwanda population with four other African populations indicated a 93% variance within populations and 7% variance among the five populations. A data base search of the 62 haplotypes yielded only one non-African haplotype match, suggesting these haplotypes are unique to the African continent.
我们使用AmpFlSTR® Yfiler™ PCR扩增试剂盒，对来自东中非卢旺达胡图族的69名无亲缘关系男性个体的群体样本中的17个Y染色体STR基因座进行了分析。在所研究的69名个体中，共鉴定出62种独特的单倍型。该群体的单倍型多样性为0.9970。基因多样性范围从0.1130（DYS392）到0.7722（DYS385）。使用主坐标分析（PCA）以及基于遗传距离矩阵的系统发育分子分析，将本研究中的群体与其他25个国家和全球群体进行比较，结果表明所有非洲群体与其他无亲缘关系的群体之间存在明显界限。群体间Fst p值的结果表明，与包括四个非洲群体在内的其他25个全球群体相比，卢旺达群体存在显著差异（p = 0.0000）。对卢旺达群体与其他四个非洲群体进行分子方差分析（AMOVA），结果显示群体内部方差占93%，五个群体之间的方差占7%。对这62种单倍型进行数据库搜索，仅发现一个与非非洲单倍型匹配的结果，这表明这些单倍型是非洲大陆特有的。
