Analytical Chemistry Division, Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Anal Bioanal Chem. 2012 Jun;403(8):2103-12. doi: 10.1007/s00216-011-5652-5.
Choline is a quaternary amine that is synthesized in the body or consumed through the diet. Choline is critical for cell membrane structure and function and in synthesis of the neurotransmitter acetylcholine. Although the human body produces this micronutrient, dietary supplementation of choline is necessary for good health. The major challenge in the analysis of choline in foods and dietary supplements is in the extraction and/or hydrolysis approach. In many products, choline is present as choline esters, which can be quantitated individually or treated with acid, base, or enzymes in order to release choline ions for analysis. A critical review of approaches based on extraction and quantitation of each choline ester as well as hydrolysis-based methods for determination of total choline in foods and dietary supplements is presented.
胆碱是一种季铵碱，可在体内合成或通过饮食摄入。胆碱对细胞膜结构和功能以及神经递质乙酰胆碱的合成至关重要。尽管人体可以产生这种微量营养素，但为了身体健康，膳食补充胆碱是必要的。分析食物和膳食补充剂中胆碱的主要挑战在于提取和/或水解方法。在许多产品中，胆碱以胆碱酯的形式存在，可将其单独定量，或用酸、碱或酶处理，以释放用于分析的胆碱离子。本文对基于每种胆碱酯提取和定量的方法以及基于水解法测定食物和膳食补充剂中总胆碱的方法进行了综述。
