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一种使用胺柱和单四极杆质谱检测器来测定饲料添加剂和饲料中氯化胆碱的直接液相色谱法。

A straightforward LC approach using an amine column and single quad mass detector to determine choline chloride in feed additives and feeds.

作者信息

Granados-Chinchilla Fabio, Artavia Graciela

机构信息

Centro de Investigación en Nutrición Animal (CINA), Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, 11501-2060 San José, Costa Rica.

Centro Nacional de Ciencia y Tecnología de Alimentos (CITA), Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, 11501-2060 San José, Costa Rica.

出版信息

MethodsX. 2017 Sep 18;4:297-304. doi: 10.1016/j.mex.2017.09.001. eCollection 2017.

DOI:10.1016/j.mex.2017.09.001
PMID:28971024
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5612802/
Abstract

Considering choline (ChCl) as an essential ingredient for animals and that it is administered through feed, we developed an easy, accurate, and sensitive method for its analysis. The method is straightforward, derivatization-free and has no secondary chromatographic interactions. •We demonstrated that the method can be used for quality control for feeds and feed additives containing choline chloride•We report a simple chromatographic method which takes advantage of the hydroxyl moiety present in ChCl and a MS detector.•We demonstrated that a single quadrupole detector is an effective option for the quantification of ChCl in feeds as an alternative for the more expensive tandem MS system.

摘要

鉴于胆碱（氯化胆碱）是动物的必需成分且通过饲料进行添加，我们开发了一种简便、准确且灵敏的分析方法。该方法操作简单、无需衍生化且不存在二次色谱相互作用。•我们证明了该方法可用于含氯化胆碱的饲料和饲料添加剂的质量控制•我们报告了一种利用氯化胆碱中存在的羟基部分和质谱检测器的简单色谱方法。•我们证明了单四极杆检测器是定量饲料中氯化胆碱的有效选择，可替代更昂贵的串联质谱系统。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ad5/5612802/42ed1f63e2b4/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ad5/5612802/ea3df67ffae2/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ad5/5612802/06ed1039deef/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ad5/5612802/7fcf5e7cf9c6/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ad5/5612802/42ed1f63e2b4/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ad5/5612802/ea3df67ffae2/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ad5/5612802/06ed1039deef/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ad5/5612802/7fcf5e7cf9c6/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ad5/5612802/42ed1f63e2b4/gr3.jpg

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引用本文的文献

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本文引用的文献

1
Determination of Free and Total Carnitine and Choline in Infant Formulas and Adult Nutritional Products by UPLC/MS/MS: Single-Laboratory Validation, First Action 2014.04.采用超高效液相色谱/串联质谱法测定婴儿配方奶粉和成人营养产品中的游离肉碱、总肉碱和胆碱：单实验室验证，2014年首次行动。
J AOAC Int. 2015 Sep-Oct;98(5):1395-406. doi: 10.5740/jaoacint.15102. Epub 2015 Jun 24.
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Analytical approaches to determination of total choline in foods and dietary supplements.食品和膳食补充剂中总胆碱的测定分析方法。
Anal Bioanal Chem. 2012 Jun;403(8):2103-12. doi: 10.1007/s00216-011-5652-5.
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Choline: an essential nutrient for public health.
胆碱：公共健康的必需营养素。
Nutr Rev. 2009 Nov;67(11):615-23. doi: 10.1111/j.1753-4887.2009.00246.x.
4
Determination of betaine, choline and trimethylamine in feed additive by ion-exchange liquid chromatography/non-suppressed conductivity detection.离子交换液相色谱/非抑制电导检测法测定饲料添加剂中的甜菜碱、胆碱和三甲胺
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