Costa Márcio Reis da, Costa Izaias Pereira da, Devalle Sylvie, Castro Ana Rita Coimbra Motta de, Freitas Solange Zacalusni
Universidade Federal de Mato Grosso.
Rev Bras Reumatol. 2012 Jan-Feb;52(1):49-54.
Recent studies on the torque teno virus (TTV), genus Anellovirus, have allowed formulating the hypothesis that TTV may trigger autoimmune rheumatic diseases or have some pathogenic role in them.
To determine the frequency of TTV infection in patients with systemic lupus erythematosus (SLE), the genetic diversity of TTV, the correlation between TTV infection and SLE clinical manifestations, and SLE clinical course and serological profile.
Serum samples were obtained from 46 SLE patients treated at the University-Affiliated Hospital of Campo Grande (NHU/FAMED/UFMS), Brazil. For controls, serum samples were obtained from 46 healthy volunteer blood donors. Viral DNA was extracted from samples using the QIAamp DNA Blood Mini Kit (QIAGEN, Hilden, Germany) and amplified using nested PCR.
Positivity for TTV was found in 17 (37%) of SLE patients and in only seven (15.2%) of the controls (z test, P = 0.03). There was no correlation between TTV infection, SLE clinical manifestations, SLE clinical course, and the serological profile of the patients evaluated.
Further studies on the presence of TTV in SLE patients are required.
最近关于细小病毒科环病毒属的转矩病毒（TTV）的研究使得人们能够提出这样的假设，即TTV可能引发自身免疫性风湿疾病或在其中发挥某种致病作用。
确定系统性红斑狼疮（SLE）患者中TTV感染的频率、TTV的基因多样性、TTV感染与SLE临床表现之间的相关性以及SLE的临床病程和血清学特征。
从巴西大坎普大学附属医院（NHU/FAMED/UFMS）接受治疗的46例SLE患者中获取血清样本。作为对照，从46名健康志愿者献血者中获取血清样本。使用QIAamp DNA Blood Mini Kit（德国希尔德的QIAGEN公司）从样本中提取病毒DNA，并使用巢式PCR进行扩增。
在17例（37%）SLE患者中发现TTV呈阳性，而在对照组中仅有7例（15.2%）呈阳性（z检验，P = 0.03）。在评估的患者中，TTV感染、SLE临床表现、SLE临床病程和血清学特征之间没有相关性。
需要对SLE患者中TTV的存在进行进一步研究。
