Casu Marco, Rivera-Ingraham Georgina A, Cossu Piero, Lai Tiziana, Sanna Daria, Dedola Gian Luca, Sussarellu Rossana, Sella Gabriella, Cristo Benedetto, Curini-Galletti Marco, García-Gómez José Carlos, Espinosa Free
Dipartimento di Zoologia e Genetica Evoluzionistica, Università di Sassari, Via F. Muroni 25, 07100 Sassari, Italy.
Genetica. 2011 Oct;139(10):1293-1308. doi: 10.1007/s10709-012-9631-3.
Patella ferruginea Gmelin, 1791 is an endangered marine gastropod endemic to the Western Mediterranean. Its range is restricted to the Sardinian-Corsican region (SCR), North Africa, a few scattered sites in Southern Spain, and Sicily. Inter-simple sequence repeat (ISSR) markers and three different mitochondrial DNA (mtDNA) regions, Cytochrome c Oxidase subunit I, 12S (small-subunit ribosomal RNA gene) and 16S (large-subunit ribosomal RNA gene), were used to investigate the presence of genetic population structuring. The mtDNA sequences showed very low levels of genetic differentiation. Conversely, ISSRs showed the presence of two main genetic groups, corresponding to Spain, North Africa and Sicily and the SCR. The SCR was further split into two subgroups. The ISSR results suggest that, on a regional scale, the genetic structure of P. ferruginea is mainly determined by the restriction of gene flow by dispersal barriers. On a more local scale human harvesting may play a crucial role in population structuring by increasing the effect of genetic drift.
地中海皱疤螺（Patella ferruginea Gmelin，1791）是一种濒危的海洋腹足纲动物，为西地中海地区特有物种。其分布范围局限于撒丁岛 - 科西嘉岛地区（SCR）、北非、西班牙南部的一些零散地点以及西西里岛。利用简单序列重复区间（ISSR）标记和三个不同的线粒体DNA（mtDNA）区域，即细胞色素c氧化酶亚基I、12S（小亚基核糖体RNA基因）和16S（大亚基核糖体RNA基因），来研究遗传种群结构的存在情况。mtDNA序列显示出极低水平的遗传分化。相反，ISSR显示存在两个主要的遗传群体，分别对应西班牙、北非和西西里岛以及SCR。SCR进一步分为两个亚组。ISSR结果表明，在区域尺度上，地中海皱疤螺的遗传结构主要由扩散障碍对基因流动的限制所决定。在更局部的尺度上，人类捕捞可能通过增加遗传漂变的影响在种群结构形成中发挥关键作用。
