Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand.
Appl Environ Microbiol. 2012 Apr;78(7):2477-81. doi: 10.1128/AEM.06951-11. Epub 2012 Jan 27.
Free ions of Na(+), K(+), Ca(2+), and Mg(2+) influenced the optical density of planktonic cultures of thermophilic bacilli. Anoxybacillus flavithermus E16 and Geobacillus sp. strain F75 (milk powder manufacturing plant isolates) and A. flavithermus DSM 2641 and G. thermoleovorans DSM 5366 were studied. Ca(2+) and Mg(2+) were associated with increases in optical density more so than Na(+) and K(+). Overall, it appeared that Ca(2+) and/or Mg(2+) was required for the production of protein in thermophilic bacilli, as shown by results obtained with A. flavithermus E16, which was selected for further study.
游离的 Na(+)、K(+)、Ca(2+) 和 Mg(2+) 离子影响嗜热杆菌浮游培养物的光密度。研究了嗜热芽孢杆菌 E16 和地芽孢杆菌 F75(奶粉制造厂分离株)以及 A. flavithermus DSM 2641 和 G. thermoleovorans DSM 5366。Ca(2+) 和 Mg(2+) 与光密度的增加比 Na(+) 和 K(+) 更相关。总体而言,嗜热杆菌产生蛋白质似乎需要 Ca(2+) 和/或 Mg(2+),这从对 A. flavithermus E16 的研究结果中可以看出,该菌被选为进一步研究的对象。