Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA, USA.
Int Forum Allergy Rhinol. 2011 Sep-Oct;1(5):361-5. doi: 10.1002/alr.20041. Epub 2011 Jul 12.
Treatment of sinonasal disease is predicated on the delivery and retention of the agent at the desired location. Despite multiple distribution studies, there remains a need for a method capable quantifying not only the site and amount of agent deposition but the rate of clearance as well.
Five patients underwent endoscopic image capture of 3 subsites over 15 minutes following fluorescein-dyed irrigation using a squeeze bottle, nasal saline spray, or nasal gel spray. Area and intensity staining were quantified using a graphics editing program. Total intensity scores were compared using a Student t test.
The squeeze bottle was the only method demonstrating delivery to the oropharynx (37 ± 22 mm(2) ). The nasal gel resulted in the greatest coverage of the middle turbinate region (10 ± 15 mm(2) ) while the nasal saline spray (75 ± 31 mm(2) ) resulted in the greatest inferior turbinate coverage. No significant differences in clearance rates were found between delivery methods at any subsite.
This method is capable of quantifying both the area and intensity of fluorescein deposition using a variety of delivery methods. While small variations in subsite coverage were noted, all methods resulted in significant internal valve deposition. Despite differences in delivery volume and viscosity, all methods resulted in similar rates of fluorescein clearance.
治疗鼻腔鼻窦疾病的前提是将药物递送到并保留在所需的位置。尽管进行了多项分布研究，但仍需要一种方法，不仅能够定量测量药物沉积的位置和数量，还能够定量测量清除率。
5 名患者在使用挤压瓶、鼻腔生理盐水喷雾或鼻腔凝胶喷雾进行荧光素染色冲洗后 15 分钟内对 3 个亚部位进行内镜图像采集。使用图形编辑程序对区域和强度染色进行量化。使用学生 t 检验比较总积分评分。
挤压瓶是唯一一种能够将药物递送到口咽部（37 ± 22 mm²）的方法。鼻凝胶可最大程度地覆盖中鼻甲区域（10 ± 15 mm²），而鼻腔生理盐水喷雾（75 ± 31 mm²）可最大程度地覆盖下鼻甲。在任何亚部位，不同给药方法之间的清除率均无显著差异。
该方法能够使用多种给药方法定量测量荧光素沉积的面积和强度。虽然注意到亚部位覆盖面积存在微小差异，但所有方法均导致明显的内阀沉积。尽管输送体积和粘度存在差异，但所有方法均导致荧光素清除率相似。
