利用中性粒细胞活性氧产物测定法测定癌症患者的慢性炎症状态。

Determination of chronic inflammatory states in cancer patients using assay of reactive oxygen species production by neutrophils.

机构信息

Cooperative Major in Advanced Health Science, Graduate School of Collaborative Education Curriculum, Tokyo University of Agriculture and Technology, Waseda University, Tokyo, [corrected] Japan.

出版信息

Anticancer Res. 2012 Feb;32(2):565-70.

PMID:22287746

Abstract

BACKGROUND

Although the systemic inflammatory condition can be confirmed in cancer patients, the pathophysiological importance of reactive oxygen species (ROS) produced by neutrophils has not yet been defined.

PATIENTS AND METHODS

Twenty-one patients with inoperable, chemoresistant and radioresistant cancer were enrolled in this study. At least 4 weeks prior to sampling, the patients were free from antitumor treatments. Control samples were also obtained from a healthy donor (39-year-old male). Peripheral blood samples were set 150 μl each on the 2 ml tube with 50 μl Mebiol Gel, and the production of ROS from neutrophils was detected by luminol-dependent chemiluminescence (LmCL) in a kinetic mode at 30-minute intervals for 2.5 hours with a luminometer at 37°C.

RESULTS

Each point, peak value and sum of values of LmCL in the patient group was statistically higher than those in the healthy donor. There were no differences in LmCL according to performance status (PS), type of cancer, age, or gender in cancer patients.

CONCLUSION

Our findings suggested that ROS produced by neutrophils universally reflects the systemic inflammatory condition in cancer patients.

摘要

背景

尽管可以在癌症患者中确认全身性炎症状态，但中性粒细胞产生的活性氧（ROS）的病理生理重要性尚未确定。

患者和方法

本研究纳入了 21 名患有无法手术、耐药和耐放射治疗的癌症的患者。在取样前至少 4 周，患者已停止抗肿瘤治疗。对照样本也取自一名健康供体（39 岁男性）。将外周血样本各设为 150μl 置于装有 50μl Mebiol Gel 的 2ml 管中，并在 37°C 下使用发光计以 30 分钟间隔的动力学模式检测中性粒细胞产生的 ROS 的发光依赖性化学发光（LmCL），持续 2.5 小时。