State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin, China.
ACS Comb Sci. 2012 Mar 12;14(3):191-6. doi: 10.1021/co2001394. Epub 2012 Feb 10.
In this paper, we demonstrate the novel use of poly(3,4-ethylene dioxythiophene) (PEDOT) nanoparticle as a very effective fluorescent sensing platform for the detection of nucleic acid sequences. The principle of the assay lies in the fact that the adsorption of the fluorescently labeled single-stranded DNA (ssDNA) probe by PEDOT nanoparticle leads to substantial fluorescence quenching, followed by specific hybridization with the complementary region of the target DNA sequence. This results in desorption of the hybridized complex from PEDOT nanoparticle surface and subsequent recovery of fluorescence. A detection limit as low as 30 pM could be achieved in this sensing system. We also demonstrate its application for multiplexed detection of nucleic acid sequences. Furthermore, this sensing system can realize the detection of single-base mismatch even in multiplexed format. It is of importance to note that the successful use of this sensing platform in human blood serum system is also demonstrated.
在本文中，我们展示了聚（3,4-亚乙基二氧噻吩）（PEDOT）纳米粒子的新颖用途，将其作为一种非常有效的荧光传感平台，用于检测核酸序列。该测定法的原理在于，荧光标记的单链 DNA（ssDNA）探针被 PEDOT 纳米粒子吸附会导致荧光显著猝灭，随后与靶 DNA 序列的互补区域进行特异性杂交。这导致杂交复合物从 PEDOT 纳米粒子表面解吸，并随后恢复荧光。在该传感系统中可以实现低至 30 pM 的检测极限。我们还展示了其在核酸序列的多重检测中的应用。此外，即使在多重检测形式下，该传感系统也能够实现单碱基错配的检测。值得注意的是，还成功地在人血清系统中使用了这种传感平台。
