State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, People's Republic of China.
Nucleic Acids Res. 2011 Mar;39(6):e37. doi: 10.1093/nar/gkq1294. Epub 2010 Dec 22.
In this article, we report on the facile and rapid synthesis of conjugation polymer poly(p-phenylenediamine) nanobelts (PNs) via room temperature chemical oxidation polymerization of p-phenylenediamine monomers by ammonium persulfate in aqueous medium. We further demonstrate the proof-of-concept that PNs can be used as an effective fluorescent sensing platform for nucleic acid detection for the first time. The general concept used in this approach lies in the facts that the adsorption of the fluorescently labeled single-stranded DNA probe by PN leads to substantial fluorescence quenching, followed by specific hybridization with the complementary region of the target DNA sequence. This results in desorption of the hybridized complex from PN surface and subsequent recovery of fluorescence. We also show that the sensing platform described herein can be used for multiplexing detection of nucleic acid sequences.
在本文中，我们报告了通过过硫酸铵在水溶液中室温化学氧化聚合对苯二胺单体，简便快速地合成共轭聚合物聚对苯二胺纳米带（PNs）。我们进一步首次证明了 PNs 可用作核酸检测的有效荧光传感平台的概念验证。该方法中使用的一般概念在于，荧光标记的单链 DNA 探针通过 PN 的吸附导致荧光显著猝灭，随后与靶 DNA 序列的互补区域特异性杂交。这导致杂交复合物从 PN 表面解吸，随后荧光恢复。我们还表明，本文所述的传感平台可用于核酸序列的多重检测。
