Liu K, Li G, Fan C, Zhou X, Wu B, Li J
Department of Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
J Int Med Res. 2011;39(6):2288-95. doi: 10.1177/147323001103900626.
The expression of microRNA-21 (miR-21) was determined in 42 patients with colorectal cancer (CRC) using real-time reverse transcription-polymerase chain reaction. The level of miR-21 in CRC tumour tissue was compared with paired normal adjacent tissue (NAT) and the relationships of miR-21 levels to clinicopathological characteristics and pathological tumour response to neoadjuvant chemotherapy were investigated. There was a significantly higher level of miR-21 in CRC tumour tissue than in NAT and high expression of miR-21 was significantly correlated with advanced clinical stage and poor cell differentiation. Receiver operating characteristic curve analysis indicated a maximum optimal cut-off cycle threshold value of 10.32 for differentiating pathological responders from non-responders, with a sensitivity of 80.0% and specificity of 88.2%. These data showed that miR-21 was significantly overexpressed in CRC tumour tissue and was associated with advanced CRC, and that miR-21 may be a potential candidate biomarker for predicting pathological tumour response to chemotherapy.
采用实时逆转录聚合酶链反应法，对42例结直肠癌（CRC）患者的微小RNA-21（miR-21）表达进行了测定。将CRC肿瘤组织中的miR-21水平与配对的正常相邻组织（NAT）进行比较，并研究miR-21水平与临床病理特征以及病理肿瘤对新辅助化疗反应之间的关系。CRC肿瘤组织中的miR-21水平显著高于NAT，且miR-21的高表达与临床晚期和细胞分化差显著相关。受试者工作特征曲线分析表明，区分病理反应者与无反应者的最大最佳截断循环阈值为10.32，敏感性为80.0%，特异性为88.2%。这些数据表明，miR-21在CRC肿瘤组织中显著过表达，并与晚期CRC相关，且miR-21可能是预测病理肿瘤对化疗反应的潜在候选生物标志物。
