Southern Regional Research Center, Agricultural Research Service, US Department of Agriculture, New Orleans, LA 70124, United States.
Fungal Biol. 2012 Feb;116(2):298-307. doi: 10.1016/j.funbio.2011.12.003. Epub 2011 Dec 17.
LaeA of Aspergillus nidulans is a putative methyltransferase and a component of the velvet complex; it is thought to mainly affect expression of genes required for the production of secondary metabolites. We found that although Aspergillus flavus CA14 laeA deletion mutants showed no aflatoxin production, expression of some of the early genes involved in aflatoxin formation, but not the later genes, could still be detected. The mutants grown in minimal medium supplemented with simple sugars and on some complex media exhibited altered conidial development. On potato dextrose agar (PDA) medium the deletion mutants showed reduced conidial chain elongation, increased production of conidiophores, and decreased colony hydrophobicity when compared to the parental strain. The loss of hydrophobicity and the other developmental changes in the laeA deletion mutants could affect the ability of the fungus to produce aflatoxins.
构巢曲霉 LaeA 是一种假定的甲基转移酶，也是绒毡层复合物的一个组成部分；它被认为主要影响次级代谢产物产生所需基因的表达。我们发现，尽管黄曲霉 CA14 的 laeA 缺失突变体不产生黄曲霉毒素，但仍能检测到一些参与黄曲霉毒素形成的早期基因的表达，而不是后期基因。在补充简单糖的最低培养基和一些复杂培养基中生长的突变体表现出改变的分生孢子发育。与亲本菌株相比，在马铃薯葡萄糖琼脂（PDA）培养基上，缺失突变体的分生孢子链伸长减少，分生孢子梗生成增加，菌落疏水性降低。LaeA 缺失突变体的疏水性丧失和其他发育变化可能会影响真菌产生黄曲霉毒素的能力。
