Department of Physiology, Chronic Inflammatory Disease Research Center, Ajou University School of Medicine, Yeongtong-gu, Suwon, Republic of Korea.
Osteoarthritis Cartilage. 2012 Apr;20(4):314-22. doi: 10.1016/j.joca.2012.01.005. Epub 2012 Jan 24.
Arthritis with intra-articular inflammation was accompanied by joint pain, swelling, and stiffness leading to significant functional impairment. Thus, regulation of joint inflammation is a good therapeutic approach for patients with arthritis. In this study, the effect of low intensity ultrasound (LIUS) applied to an adjuvant-induced arthritic rat model on the synovium was investigated.
Synovial inflammation was induced by complete Freund's adjuvant (CFA)-injection into the rat knee joint. LIUS (200 mW/cm(2)) was applied on the ipsilateral knee everyday for 10 min beginning 1 day after inflammation induction. The expression of proinflammatory factors and immunohistochemical staining pattern of the synovium were assessed.
CFA induced an increase of the knee circumference that was significantly diminished by LIUS. Synovial membrane hyperplasia in the ipsilateral joint was also affected by LIUS. The inflammatory mediators, COX-1/2, IL-1β, and iNOS, but not TNF-α, in the synovial membrane were induced after 3 days, and they closely correlated with the degree of edema. In the synovial membrane, the expression of inflammatory mediators was reduced by LIUS. The chemoattractant chemokine receptor CCR5 also was involved. On immunohistochemical analysis, CFA caused increased infiltration of CD11b-positive cells in the synovium. After 3 days, neutrophils, myeloperoxidase (MPO)-positive cells filled the inflammatory core; later, monocytes and macrophages, ionized calcium binding adaptor molecule 1 (Iba1)-positive cells in the periphery infiltrated the core by day 5. LIUS markedly reduced CFA-induced inflammatory cells infiltration.
LIUS showed a potent anti-inflammatory effect in this animal arthritis model with reduced infiltration of inflammatory cells into the synovium.
伴有关节内炎症的关节炎伴有关节疼痛、肿胀和僵硬，导致明显的功能障碍。因此，调节关节炎症是关节炎患者的一种良好治疗方法。在这项研究中，研究了低强度超声（LIUS）应用于佐剂诱导的关节炎大鼠模型对滑膜的影响。
通过向大鼠膝关节注射完全弗氏佐剂（CFA）诱导滑膜炎症。LIUS（200 mW/cm²）在炎症诱导后第 1 天开始每天应用于对侧膝关节 10 分钟，共 10 天。评估了促炎因子的表达和滑膜的免疫组织化学染色模式。
CFA 诱导的膝关节周长增加，LIUS 显著减少。LIUS 还影响同侧关节滑膜膜增生。炎症介质 COX-1/2、IL-1β 和 iNOS，但不是 TNF-α，在滑膜中诱导 3 天后，与水肿程度密切相关。在滑膜中，LIUS 减少了炎症介质的表达。趋化因子受体 CCR5 也参与其中。在免疫组织化学分析中，CFA 导致滑膜中 CD11b 阳性细胞的浸润增加。3 天后，中性粒细胞、髓过氧化物酶（MPO）阳性细胞充满炎症核心；随后，单核细胞和巨噬细胞、离子钙结合衔接分子 1（Iba1）阳性细胞在第 5 天向核心浸润。LIUS 显著减少了 CFA 诱导的炎症细胞浸润。
LIUS 在这种动物关节炎模型中表现出强大的抗炎作用，减少了炎症细胞向滑膜的浸润。
