大剂量地塞米松对调节免疫性血小板减少症中白细胞介素-22 产生及纠正 Th1 和 Th22 极化的影响。
Effects of high-dose dexamethasone on regulating interleukin-22 production and correcting Th1 and Th22 polarization in immune thrombocytopenia.
机构信息
Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, No. 99 West Huaihai Road, Xuzhou 221002, China.
出版信息
J Clin Immunol. 2012 Jun;32(3):523-9. doi: 10.1007/s10875-012-9649-4.
BACKGROUND
T-cell dysregulation and T-cell-related cytokine abnormalities are involved in the pathogenesis of immune thrombocytopenia (ITP). One of our previous studies showed that elevated IL-22 correlated to Th1 and Th22 cells plays an important role in the immunopathogenesis of ITP. In this study, we aimed to investigate the effects of high-dose dexamethasone(HD-DXM) on IL-22 production and on the IL-22-producing T-cell subsets in ITP patients.
METHODS
IL-22 plasma levels and the percentages of Th1, Th17, and Th22 cells were determined by enzyme-linked immunosorbent assay and flow cytometry in 25 ITP patients receiving DXM 40 mg/day for 4 consecutive days.
RESULTS
Plasma IL-22 concentrations and the percentages of Th1 and Th22 cells were significantly increased in pretherapy patients relative to controls (P<0.05), but the percentage of Th17 cells was not. HD-DXM administration reduced IL-22 production and corrected the imbalance between Th1 and Th22 subsets. IL-22 levels were positively correlated with Th1 and Th22 cells in ITP patients before and after HD-DXM treatment.
CONCLUSION
These results suggest that HD-DXM may regulate the production of IL-22 in ITP, possibly by correcting Th1 and Th22 polarization.
背景
T 细胞失调和 T 细胞相关细胞因子异常参与了免疫性血小板减少症（ITP）的发病机制。我们之前的一项研究表明，升高的 IL-22 与 Th1 和 Th22 细胞相关，在 ITP 的免疫发病机制中发挥重要作用。在这项研究中，我们旨在研究大剂量地塞米松（HD-DXM）对 ITP 患者 IL-22 产生及其产生的 IL-22 阳性 T 细胞亚群的影响。
方法
通过酶联免疫吸附试验和流式细胞术，检测 25 例接受 DXM 40mg/d，连续 4 天治疗的 ITP 患者的 IL-22 血浆水平和 Th1、Th17 和 Th22 细胞的比例。
结果
与对照组相比，治疗前患者的血浆 IL-22 浓度和 Th1 和 Th22 细胞的比例明显升高（P<0.05），但 Th17 细胞的比例没有升高。HD-DXM 治疗可降低 IL-22 的产生，并纠正 Th1 和 Th22 亚群之间的失衡。在接受 HD-DXM 治疗前后，ITP 患者的 IL-22 水平与 Th1 和 Th22 细胞呈正相关。
结论
这些结果表明，HD-DXM 可能通过纠正 Th1 和 Th22 极化来调节 ITP 中 IL-22 的产生。
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