酿酒酵母的性反应：经交配因子处理的细胞提取物对酵母乙二醛酶I的磷酸化作用。
Sexual response of Saccharomyces cerevisiae: phosphorylation of yeast glyoxalase I by a cell extract of mating factor-treated cells.
作者信息
Inoue Y, Choi B Y, Murata K, Kimura A
机构信息
Research Institute for Food Science, Kyoto University.
出版信息
J Biochem. 1990 Jul;108(1):4-6. doi: 10.1093/oxfordjournals.jbchem.a123159.
Abstract
The phosphorylation of glyoxalase I was observed when the phosphatase-treated enzyme was incubated in the presence of [gamma-32P]ATP and a cell extract prepared from alpha-type yeast cells which had been treated with the culture supernatant of a-type yeast cells. The phosphorylated protein was identified as glyoxalase I by using anti-glyoxalase I rabbit immunoglobulin G.
摘要
当用磷酸酶处理过的乙二醛酶I在[γ-32P]ATP以及由经a型酵母细胞培养上清液处理过的α型酵母细胞制备的细胞提取物存在的情况下进行孵育时，可观察到乙二醛酶I发生磷酸化。通过使用抗乙二醛酶I兔免疫球蛋白G，将磷酸化的蛋白质鉴定为乙二醛酶I。
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