Das L K, Pani S P, Vanamail P, Vijayalakshmi G, Debritto L J
Vector Control Research Centre (VCRC), Indian Council of Medical Research (ICMR), Pondicherry, India.
Eur J Clin Microbiol Infect Dis. 2012 Jan 31. doi: 10.1007/s10096-011-1542-1.
This study was focussed on identifying a cost-effective method for delimitation, monitoring and evaluation in bancroftian filariasis. Finger prick blood samples were collected between 20.00 and 23.00 hours for the detection of microfilariae (mf) from the available population in a village which was endemic for lymphatic filariasis. Simultaneously, from each individual, four spots of 25-μl blood samples were collected on Whatman number 3 filter paper and air dried. Dried filter paper spots were pooled in quantities of 1, 5, 10, 15, 20 and 25 on unknown and simulated mf and antigen prevalence. Pooled samples were assayed for circulating filarial antigen (CFA) using TropBIO Og4C3 ELISA kits. The community mf and CFA rates were 3.4% and 25.9%, respectively. The pool sizes of 20 and 25 showed CFA positivity in all the above categories tested. The results of the pooled blood spot samples suggest that, in areas with mf and CFA prevalence rates between 1 and 10%, pools of 20 or 25 could be considered as the ideal pool size for the detection of filarial infection in the community. CFA prevalence at the level of 5-6% following desirable rounds of mass drug administration (MDA) indicates that the community mf prevalence is likely to be at the 1% level.
本研究着重于确定一种用于班氏丝虫病划界、监测和评估的经济有效方法。在一个淋巴丝虫病流行的村庄，于20:00至23:00采集手指刺血样本，以检测现有居民中的微丝蚴（mf）。同时，从每个个体采集25微升血样，在Whatman 3号滤纸上点四个点，然后风干。将干燥的滤纸血点按数量1、5、10、15、20和25合并，用于未知和模拟的mf及抗原流行率检测。使用TropBIO Og4C3 ELISA试剂盒对合并样本进行循环丝虫抗原（CFA）检测。社区mf和CFA感染率分别为3.4%和25.9%。20和25的合并样本量在所有上述检测类别中均显示CFA呈阳性。合并血斑样本的结果表明，在mf和CFA流行率为1%至10%的地区，20或25的合并样本量可被视为社区丝虫感染检测的理想合并样本量。在进行理想轮次的大规模药物治疗（MDA）后，CFA流行率达到5%至6%，这表明社区mf流行率可能处于1%的水平。
