Rocha Abraham, Lima Guilherme, Medeiros Zulma, Aguiar-Santos Ana, Alves Sandra, Montarroyos Ulisses, Oliveira Paula, Béliz Fátima, Netto Maria José, Furtado André
Departamento de Parasitologia, Serviço de Referência Nacional em Filariose, Centro de Pesquisas Aggeu Magalhães, Fiocruz, Recife, PE, Brasil.
Mem Inst Oswaldo Cruz. 2004 Feb;99(1):101-5. doi: 10.1590/s0074-02762004000100018. Epub 2004 Mar 31.
The purpose of this study was to examine the circulating filarial antigen (CFA) detected by the monoclonal antibody (mAb) Og4C3-ELISA in paired samples of serum and hydrocele fluid from 104 men with hydrocele, living in an endemic area of Wuchereria bancrofti. Nocturnal blood specimens were filtered and examined for microfilariae (MF) and ultrasound was used in order to identify the presence of adult worms (the filaria dance sign - FDS) in the lymphatic vessels of the scrotal area. Four groups were selected according to their parasitological status: group I - 71 MF- and FDS-; group II - 21 MF+ and FDS+; group III - 10 MF- and FDS+ and group IV- 2 MF+ and FDS-. CFA was identified simultaneously (fluid and serum) in 11 (15.5%), 21 (100%), 3 (30%), and 1 (50%) in groups I, II, III, and IV, respectively. In despite of high CFA+ level (antigen Og4C3) units/ml, the Geometrical Mean (GM) = 2696) in the sera of these 36/104 paired samples, when compared to the hydrocele fluid, (GM = 1079), showed a very good correlation between the CFA level in the serum and CFA level in the fluid (r = 0.731). CFA level in the serum of the 23 microfilaremics (groups II and IV) was extremely high (GM = 4189) and was correlated with MF density (r = 0.442). These findings report for the first time the potential alternative use of the hydrocele fluid to investigate CFA using the mAb Og4C3-ELISA.
本研究旨在通过单克隆抗体（mAb）Og4C3-ELISA检测，对104名居住在班氏丝虫病流行区、患有鞘膜积液的男性患者的血清和鞘膜积液配对样本中的循环丝虫抗原（CFA）进行检测。夜间采集血液样本，过滤后检查微丝蚴（MF），并使用超声检查阴囊区域淋巴管中是否存在成虫（丝虫舞动征 - FDS）。根据寄生虫学状态将患者分为四组：第一组 - 71例MF-和FDS-；第二组 - 21例MF+和FDS+；第三组 - 10例MF-和FDS+；第四组 - 2例MF+和FDS-。分别在第一、二、三、四组中，同时在（积液和血清中）检测到CFA的比例为11例（15.5%）、21例（100%）、3例（30%）和1例（50%）。尽管在这104对配对样本中的36例血清中CFA+水平较高（抗原Og4C3单位/ml，几何均值（GM） = 2696），但与鞘膜积液相比（GM = 1079），血清中CFA水平与积液中CFA水平之间显示出非常好的相关性（r = 0.731）。23例微丝蚴血症患者（第二组和第四组）血清中的CFA水平极高（GM = 4189），且与MF密度相关（r = 0.442）。这些发现首次报道了使用mAb Og4C3-ELISA检测鞘膜积液用于研究CFA的潜在替代用途。
