Division of Molecular and Cellular Science, Centre for Biomolecular Science, School of Pharmacy, University of Nottingham, Nottingham, UK.
Magn Reson Chem. 2011 Dec;49 Suppl 1:S27-36. doi: 10.1002/mrc.2846.
Grapes and related products, such as juices, and in particular, their polyphenols, have previously been associated with many health benefits, such as protection against cardiovascular disease. Within grapes, a large range of structurally diverse polyphenols can be present, and their characterisation stands as a challenge. (1)H NMR spectroscopy in principle would provide a rapid, nondestructive and straightforward method for profiling of polyphenols. However, polyphenol profiling and identification in grape juices is hindered because of signals of prevailing carbohydrates causing spectral overlap and compromising dynamic range. This study describes the development of an extraction method prior to analysis using (1)H NMR spectroscopy, which can, potentially, significantly increase the number of detectable polyphenols and aid their identification, by reduction of signal overlap and selective removal of heavily dominating compounds such as sugars.
葡萄及相关产品，如葡萄汁，特别是它们的多酚类物质，此前已被证实对多种健康有益，如预防心血管疾病。在葡萄中，存在大量结构多样的多酚类物质，对其进行特征描述是一个挑战。（1）H 核磁共振（NMR）光谱法在原则上提供了一种快速、无损和直接的多酚类物质分析方法。然而，由于主要的碳水化合物信号导致光谱重叠和动态范围受限，葡萄汁中的多酚类物质分析和鉴定受到阻碍。本研究描述了在使用（1）H NMR 光谱法分析之前的提取方法的开发，该方法通过减少信号重叠和选择性去除如糖等大量存在的化合物，可能会显著增加可检测多酚类物质的数量，并有助于它们的鉴定。
