Atoum Dana, Fernandez-Pastor Ignacio, Young Louise, Edrada-Ebel RuAngelie
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, UK.
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, The Hashemite University, Zarqa 13133, Jordan.
Plants (Basel). 2023 Jun 12;12(12):2297. doi: 10.3390/plants12122297.
Plants from the Asteraceae family were commonly used to treat various diseases. The metabolomic profile of this family consisted of bioactive flavonoids and other phenolics. Chamomile is a member of the Asteraceae family. Jordanian and European chamomile are two varieties of (German chamomile), which were grown under different environmental conditions, were studied. Many examples of plant varieties with significant distinction in the secondary metabolite they afford have been described in the literature. Multivariate statistical analysis was employed to measure the depth of this variation in two chamomile varieties.
From both types, crude extracts were prepared using solvents of different polarities and tested for their biological activity. The semipolar fraction of the European variety showed anticancer and antioxidant activity. Meanwhile, the semipolar fraction of the Jordanian type exhibited only antioxidant activity. Both extracts were fractionated, and then the biological activity was again assayed.
European and Jordanian chamomile fractions produced dicaffeoylquinic acid isomers exhibiting antioxidant capability. Additionally, -glucoferulic acid was produced from the European chamomile, demonstrating antioxidant activity. The European samples afforded two major compounds, chrysosplenetin and apigenin, that displayed anticancer activity.
Different environmental conditions between Jordanian and European chamomile affected the type of isolated compounds. Structure elucidation was performed with HPLC-MS coupled with dereplication techniques and 2D NMR experiments.
菊科植物常用于治疗各种疾病。该科植物的代谢组由生物活性黄酮类化合物和其他酚类物质组成。洋甘菊是菊科植物的一员。对在不同环境条件下生长的约旦洋甘菊和欧洲洋甘菊（德国洋甘菊的两个变种）进行了研究。文献中已描述了许多在其次生代谢产物方面有显著差异的植物变种实例。采用多元统计分析来衡量这两个洋甘菊变种中这种差异的程度。
从这两种洋甘菊中，使用不同极性的溶剂制备粗提物，并测试其生物活性。欧洲变种的半极性部分显示出抗癌和抗氧化活性。同时，约旦变种的半极性部分仅表现出抗氧化活性。对两种提取物进行分馏，然后再次测定生物活性。
欧洲和约旦洋甘菊馏分产生了具有抗氧化能力的二咖啡酰奎宁酸异构体。此外，欧洲洋甘菊产生了表现出抗氧化活性的β - 葡萄糖阿魏酸。欧洲样品提供了两种具有抗癌活性的主要化合物，金缕梅素和芹菜素。
约旦洋甘菊和欧洲洋甘菊之间不同的环境条件影响了分离出的化合物类型。通过HPLC - MS结合去重技术和二维核磁共振实验进行结构解析。
