Department of Horticulture, Cornell University, Ithaca, New York 14853, United States.
J Agric Food Chem. 2012 Feb 8;60(5):1291-9. doi: 10.1021/jf2046637. Epub 2012 Jan 26.
The composition and content of polyphenols in the seeds of 91 grape accessions from 17 Vitis species were characterized. Eleven compounds, including 2 gallic derivatives, 3 monomeric flavan-3-ols, 3 flavonols, resveratrol, and procyanidin B1 and B2, were identified via HPLC-MS and quantified by HPLC-DAD. In addition, seventeen dimeric and trimeric flavan-3-ols were also quantified. Tremendous variation was observed both among and within species for these compounds. Monomeric flavan-3-ols were the most abundant polyphenols in seeds, followed by dimeric and trimeric flavan-3-ols, which collectively accounted for more than 96% of the total polyphenols. V. palmata, V. vinifera, and V. vulpina had significantly higher content of total polyphenols than other species. A number of Vitis accessions with high content of various types of seed polyphenols were identified, and they can serve as potential germplasm for improving the composition and content of seed polyphenols in cultivated grapes.
本研究对来自 17 种葡萄属(Vitis)的 91 个葡萄品种的种子中多酚的组成和含量进行了分析。通过高效液相色谱-质谱联用仪(HPLC-MS)鉴定并通过高效液相色谱-二极管阵列检测法(HPLC-DAD)进行定量分析，共鉴定出 11 种化合物，包括 2 种没食子酸衍生物、3 种单体黄烷-3-醇、3 种黄酮醇、白藜芦醇和原花青素 B1 和 B2。此外，还对 17 种二聚体和三聚体黄烷-3-醇进行了定量分析。这些化合物在种间和种内均表现出巨大的差异。单体黄烷-3-醇是种子中含量最丰富的多酚，其次是二聚体和三聚体黄烷-3-醇，它们共同占总多酚的 96%以上。 palmsata、vinifera 和 vulpina 葡萄属的总多酚含量明显高于其他种。本研究还鉴定出了一些具有高含量各种类型种子多酚的葡萄品种，它们可以作为提高栽培葡萄种子多酚组成和含量的潜在种质资源。
