Martindale V E, Salisbury J L
Laboratory for Cell Biology, Case Western Reserve University, Cleveland, OH 44118.
J Cell Sci. 1990 Jul;96 ( Pt 3):395-402. doi: 10.1242/jcs.96.3.395.
Centrin, a calcium-sensitive contractile phosphoprotein, is the major component of the striated flagellar roots of the flagellate green alga, Tetraselmis striata. Flagellar roots contract in response to elevated calcium levels. Data presented here indicate that the level of centrin phosphorylation is rapidly responsive to changes in the cell's external environment. Centrin is dephosphorylated in response to elevated calcium or to heat shock. An increase in centrin phosphorylation accompanies pH shock or ethanol treatment. These changes are compared with flagellar excision, flagellar root contraction, and protein synthesis under the same treatments. We conclude that under certain conditions phosphorylation/dephosphorylation of centrin and extension/contraction of the flagellar root are uncoupled. Possible role(s) of centrin phosphorylation independent of force generation in the flagellar root are discussed.
中心蛋白是一种对钙敏感的收缩性磷蛋白，是鞭毛绿藻条纹四鞭藻横纹鞭毛根部的主要成分。鞭毛根部会因钙水平升高而收缩。此处呈现的数据表明，中心蛋白的磷酸化水平对细胞外部环境的变化有快速反应。中心蛋白会因钙水平升高或热休克而发生去磷酸化。pH值冲击或乙醇处理会伴随中心蛋白磷酸化增加。将这些变化与相同处理下的鞭毛切除、鞭毛根部收缩和蛋白质合成进行了比较。我们得出结论，在某些条件下，中心蛋白的磷酸化/去磷酸化与鞭毛根部的伸展/收缩是解偶联的。讨论了中心蛋白磷酸化在鞭毛根部独立于力产生的可能作用。
