高效液相色谱法分离神经酰胺中的抗病毒和免疫刺激组分。

High-performance liquid chromatographic approach to the separation of antiviral and immunostimulant fractions in Neuramide.

作者信息

Miraglia N, Rindone B, Folchitto G, Amicucci P, Antonelli G, Lanfranchi A, Massa M

机构信息

Dipartimento di Chimica Organica e Industriale, Università di Milano, Italy.

出版信息

J Chromatogr. 1990 Jul 20;512:123-9. doi: 10.1016/s0021-9673(01)89478-4.

DOI:10.1016/s0021-9673(01)89478-4

PMID:2229223

Abstract

Neuramide, a tissue extract having antiviral action against influenza A virus and immunostimulant action, was analyzed by preparative size-exclusion high-performance liquid chromatography (HPLC) and a low-molecular-weight fraction responsible for the antiviral action was isolated after reversed-phase HPLC. Four fractions having immunostimulant activity were also isolated, as evidenced by their potentiating action in the human lymphocyte proliferation induced by phytohaemagglutinin.

摘要

神经酰胺是一种对甲型流感病毒具有抗病毒作用和免疫刺激作用的组织提取物，通过制备型尺寸排阻高效液相色谱（HPLC）进行分析，并在反相HPLC后分离出负责抗病毒作用的低分子量级分。还分离出了四个具有免疫刺激活性的级分，这通过它们对植物血凝素诱导的人淋巴细胞增殖的增强作用得到证明。