Zerial A, Skerlavaj B, Gennaro R, Romeo D
Rhône Poulenc Santé, Centre de Recherches de Vitry, Vitry-sur-Seine, France.
Antiviral Res. 1987 Jul;7(6):341-52. doi: 10.1016/0166-3542(87)90016-7.
From an acid extract of granules of bovine neutrophils we isolated fractions of cationic proteins, exhibiting significant anti-herpesvirus activity at concentrations which were devoid of cytotoxicity and of activity against a picornavirus (rhinovirus). The mechanism of action seems to involve a direct neutralization of the virions. Two antiviral peptides with an approximate MW 7500 were purified to homogeneity by reversed phase high-performance liquid chromatography. Proline and arginine accounted for about 43% and 26-27% of their amino acid residues. One of these peptides (IIIa2 beta) had an MIC of 2 microM.
从牛嗜中性粒细胞颗粒的酸性提取物中，我们分离出了阳离子蛋白组分，这些组分在无细胞毒性且对小核糖核酸病毒（鼻病毒）无活性的浓度下，表现出显著的抗疱疹病毒活性。其作用机制似乎涉及对病毒粒子的直接中和。通过反相高效液相色谱法将两种分子量约为7500的抗病毒肽纯化至同质。脯氨酸和精氨酸分别约占其氨基酸残基的43%和26 - 27%。其中一种肽（IIIa2β）的最低抑菌浓度为2微摩尔。
